{ "id": "ep0t8j0r39", "slug": "bioid", "title": "BioID", "updated": "2026-10-15", "topic_path": [ { "id": "life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/topics/life" }, { "id": "life.biology", "label": "Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology" }, { "id": "life.biology.biochemistry", "label": "Biochemistry and metabolism", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.biochemistry" }, { "id": "life.biology.biochemistry.practice", "label": "Biochemistry field and methods", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.biochemistry.practice" }, { "id": "life.biology.biochemistry.practice.methods", "label": "Biochemical methods and techniques", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.biochemistry.practice.methods" } ], "geo": [ { "id": "geo.nongeo.t2001.life.biology", "label": "Non-geographic · 2001 to 2020: Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.biology", "path": [ { "id": "geo.nongeo", "label": "Non-geographic", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo" }, { "id": "geo.nongeo.t2001", "label": "Non-geographic · 2001 to 2020", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001" }, { "id": "geo.nongeo.t2001.life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life" }, { "id": "geo.nongeo.t2001.life.biology", "label": "Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.biology" } ] } ], "excerpt": "BioID, or proximity-dependent biotin identification, is a method for mapping proteins near a chosen bait protein in living cells, introduced by Kyle J. Roux and colleagues in 2012.", "snippet": "BioID, or proximity-dependent biotin identification, is a method for mapping proteins near a chosen bait protein in living cells, introduced by Kyle J. Roux and colleagues in 2012.", "node": "life.biology.biochemistry.practice.methods", "markdown": "# BioID\n\nBioID (proximity-dependent biotin identification) is a method for mapping the proteins that surround a chosen bait protein in living cells. A promiscuous biotin ligase fused to the bait biotinylates nearby proteins, which are captured on streptavidin (bacterial protein binding biotin extremely tightly) and identified by mass spectrometry. The readout is a list of proximal proteins, not a set of validated direct binders; the method's authors state it should be used as a screen for candidate interactors rather than to validate physical interactions.[1](https://doi.org/10.1083/jcb.201112098) Because labeling occurs in living cells over hours, BioID records a history of proximity and can detect weak or transient associations that yeast two-hybrid and affinity purification miss.[2](https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpcb.11)\n\n| Key fact | Value |\n|---|---|\n| Enzyme | BirA*, the R118G mutant of E. coli biotin ligase, 35 kDa [1](https://doi.org/10.1083/jcb.201112098) • [3](https://link.springer.com/article/10.1186/s12964-023-01310-1) |\n| Biotin pulse | 50 µM added to medium; 18 h in the nuclear pore study and 24 h in a 2024 automated workflow for BirA* [1](https://doi.org/10.1083/jcb.201112098) • [4](https://doi.org/10.1073/pnas.1406459111) • [5](https://www.sciencedirect.com/org/science/article/pii/S1535390724003597) |\n| Effective labeling radius | ~10 nm in vivo, measured with the Nup107-160 complex as a molecular ruler [4](https://doi.org/10.1073/pnas.1406459111) |\n| Fastest common ligases | TurboID and miniTurbo label within 10 min [6](https://doi.org/10.1038/nbt.4201) |\n| Biotin–streptavidin affinity | Reported as \\( K_{\\mathrm{d}} \\approx 10^{-14} \\) M in one review [7](https://pmc.ncbi.nlm.nih.gov/articles/PMC7196579/) and \\( K_{\\mathrm{d}} \\approx 10^{-14} \\) M in a 2024 methods paper [5](https://www.sciencedirect.com/org/science/article/pii/S1535390724003597) |\n| Scoring | SAINT or SAINTexpress against localization-matched controls, with CRAPome for contaminants [8](https://www.sciencedirect.com/science/article/pii/S2666166721007814) |\n\n## How it works\n\nWild-type E. coli biotin protein ligase (BirA) synthesizes the reactive intermediate biotinoyl-5'-AMP and transfers biotin onto a specific carrier protein. The R118G mutation (BirA*) lowers the enzyme's affinity for biotinoyl-5'-AMP by two orders of magnitude, so the intermediate is released prematurely and reacts with primary amines, chiefly exposed lysine residues, on whatever proteins sit nearby.[1](https://doi.org/10.1083/jcb.201112098) One review quantifies the defect as a 100-fold greater \\( K_{\\mathrm{d}} \\) for biotin and a 400-fold higher dissociation rate for biotinyl-5'-AMP [7](https://pmc.ncbi.nlm.nih.gov/articles/PMC7196579/), while another reports approximately 40-fold and 440-fold reductions in affinity for biotin and bioAMP respectively; the two accounts disagree and the discrepancy is unresolved.[3](https://link.springer.com/article/10.1186/s12964-023-01310-1) Adding 50 µM biotin to the culture medium strongly stimulates promiscuous biotinylation by BirA* but not by wild-type BirA.[1](https://doi.org/10.1083/jcb.201112098)\n\nThe initial paper estimated that roughly half of detected proteins lie within about 20–30 nm of the bait, based on nuclear lamina dimensions.[1](https://doi.org/10.1083/jcb.201112098) A later study using the Nup107-160 Y-complex as a molecular ruler put the practical in vivo radius at ~10 nm.[4](https://doi.org/10.1073/pnas.1406459111) In 2025, DNA nanorulers carrying oligonucleotide-barcoded targets at nanometer precision showed that TurboID labels primarily through direct contact rather than the prevailing diffusive model, while APEX2 combines high contact-range efficiency with low-level diffusive labeling.[9](https://www.nature.com/articles/s41589-025-02086-w)\n\n## How it is done\n\nA typical experiment proceeds in five stages. First, the bait is cloned in-frame with BirA* (or a faster ligase) and the fusion's expression level and subcellular localization are verified. Second, expression is induced, for example with tetracycline at 1 µg/mL.[8](https://www.sciencedirect.com/science/article/pii/S2666166721007814) Third, biotin is added: 50 µM for 18 h with BirA* in the nuclear pore study and 24 h in a 2024 automated workflow, versus 10 min for TurboID or miniTurbo and 3 h for AirID.[4](https://doi.org/10.1073/pnas.1406459111) • [5](https://www.sciencedirect.com/org/science/article/pii/S1535390724003597) • [8](https://www.sciencedirect.com/science/article/pii/S2666166721007814) Fourth, cells are lysed under harsh conditions, sonicated, and incubated with streptavidin-coated Sepharose; the very high biotin–streptavidin affinity permits stringent washing, though on-bead trypsin digestion generates abundant streptavidin peptide contamination.[7](https://pmc.ncbi.nlm.nih.gov/articles/PMC7196579/) • [5](https://www.sciencedirect.com/org/science/article/pii/S1535390724003597) • [10](https://www.protocols.io/view/bioid-identifying-protein-protein-interactions-in-dd8729zn.pdf) Fifth, eluted peptides are identified by LC-MS/MS, for example by data-independent acquisition.[10](https://www.protocols.io/view/bioid-identifying-protein-protein-interactions-in-dd8729zn.pdf)\n\nControls are essential because streptavidin pulls down endogenously biotinylated enzymes and other background. Controls should match the bait's localization, such as NLS-tagged BirA* for a nuclear bait or EGFP-CAAX-BirA* for a membrane bait, alongside empty-vector controls and at least two biological replicates; candidates are scored with SAINT or SAINTexpress through ProHits and filtered against the CRAPome contaminant repository.[8](https://www.sciencedirect.com/science/article/pii/S2666166721007814) An automated Agilent Bravo workflow with acetylated streptavidin beads, on-bead LysC digestion, and two-step elution identified 80 protein groups matching known interactors versus 44 manually, quantified 2740 more protein groups, and improved the F1 separation score from 0.903 to 0.955.[5](https://www.sciencedirect.com/org/science/article/pii/S1535390724003597)\n\n## Origin\n\nBioID was introduced by Kyle J. Roux and colleagues in the Journal of Cell Biology in 2012.[1](https://doi.org/10.1083/jcb.201112098) Two earlier findings made it possible: E. coli biotin protein ligase promiscuously biotinylates proteins in vitro [11](https://doi.org/10.1110/ps.04911804), and Kwon and Beckett had characterized the R118G mutation's reduced bioAMP affinity in 2000.[12](https://doi.org/10.1110/ps.9.8.1530) The design was modeled on DamID, an enzyme-fusion approach for tracing DNA contacts.[1](https://doi.org/10.1083/jcb.201112098) Applied to lamin A, BioID recovered known nuclear envelope interactors and a previously uncharacterized NE-associated protein, SLAP75, demonstrating its use for insoluble nuclear proteins poorly served by affinity methods.[1](https://doi.org/10.1083/jcb.201112098) • [2](https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpcb.11)\n\n## Variants\n\nBioID2, introduced by Dae In Kim and colleagues in 2016, uses a smaller (27 kDa) biotin ligase from Aquifex aeolicus and requires less supplementary biotin than the 35 kDa BirA*.[13](https://doi.org/10.1091/mbc.e15-12-0844) • [3](https://link.springer.com/article/10.1186/s12964-023-01310-1) TurboID (35 kDa) and miniTurbo (28 kDa) were engineered by Tess C. Branon and colleagues in 2018 through yeast display-based directed evolution; they carry 15 and 12 mutations relative to wild-type BirA respectively (miniTurbo also deletes the N-terminal 63 amino acids), deliver in 10 min what BioID or BioID2 deliver in over 18 h, function at lower temperatures, and extend labeling to flies and worms.[6](https://doi.org/10.1038/nbt.4201) • [7](https://pmc.ncbi.nlm.nih.gov/articles/PMC7196579/) • [3](https://link.springer.com/article/10.1186/s12964-023-01310-1) Split-BioID is a variant in which BirA* fragments reassemble only when two proteins interact.[14](https://doi.org/10.1002/1873-3468.12548) • [15](https://doi.org/10.1038/ncomms15690) Split-TurboID, from Cho and colleagues in 2020, was derived by testing 14 split points; after rapamycin-dependent reconstitution through FKBP-FRB it labels with under 1 h of biotin, with activity exceeding split-BioID and full-length BioID.[16](https://doi.org/10.1073/pnas.1919528117) • [3](https://link.springer.com/article/10.1186/s12964-023-01310-1) AirID, introduced by Kido and colleagues in 2020, is a lower-background ligase used with 3 h labeling.[17](https://doi.org/10.7554/elife.54983) • [8](https://www.sciencedirect.com/science/article/pii/S2666166721007814) Two-component designs followed, including 2C-BioID.[18](https://doi.org/10.1016/j.isci.2018.11.023)\n\n## Applications\n\nBioID suits questions where interactions are transient, insoluble, or spatially organized. Founding applications mapped the nuclear lamina and nuclear pore complex architecture.[1](https://doi.org/10.1083/jcb.201112098) • [4](https://doi.org/10.1073/pnas.1406459111) Split-TurboID reports labeling only where two proteins contact.[16](https://doi.org/10.1073/pnas.1919528117) TurboID's speed enables proximity labeling in whole flies and worms.[6](https://doi.org/10.1038/nbt.4201) Fusing dCas9 to TurboID or UltraID (CasTurbo and CasUltra) brings labeling to specific genomic loci; CasUltra applied to A375 melanoma cells quantified approximately 2000 proteins per sample and profiled MYC promoter reorganization after JQ1 BET inhibition.[19](https://pubs.acs.org/jprobs/article-lookup/doi/10.1021/acs.jproteome.4c00931) STUPPIT, a 2025 two-step scheme combining split-TurboID with PUP-IT, labels intermediary proteins bridging two non-interacting proteins, and identified ERC1 and USP7 as novel β-catenin/SMAD4 intermediaries.[20](https://journals.plos.org/plosbiology/article?id=10.1371%2Fjournal.pbio.3003227)\n\n## Limitations and alternatives\n\nBioID measures proximity, not binding: results require validation by co-immunoprecipitation, pull-down, BRET, yeast two-hybrid, or split GFP.[8](https://www.sciencedirect.com/science/article/pii/S2666166721007814) Transient overexpression of fusion proteins can cause mislocalization, aggregation, and artificial interactions; endogenous CRISPR knock-in of TurboID at the AP-1 adaptor µ subunit revealed known interactors and cargo proteins that overexpression could not, and moved from CRISPR transfection to interactome data in just over a month.[21](https://pubmed.ncbi.nlm.nih.gov/39056144/) TurboID's high biotin affinity lets it use biotin in serum, causing background labeling, and constitutive TurboID expression in fly tissues depletes biotin, reducing survival and body size; labeling beyond 24 h in cultured cells causes overbiotinylation and growth defects.[7](https://pmc.ncbi.nlm.nih.gov/articles/PMC7196579/) • [3](https://link.springer.com/article/10.1186/s12964-023-01310-1) Because labeling depends on surface-exposed lysines and biotinylated peptides stay attached to streptavidin after trypsin digestion, low molecular weight proteins are underdetected, and baits with less confined localization yield more likely false positives.[22](https://pmc.ncbi.nlm.nih.gov/articles/PMC9791439/)\n\nCompared with APEX2, an engineered ascorbate peroxidase originally developed for electron microscopy [23](https://doi.org/10.1038/nbt.2375) and evolved for proximity labeling [24](https://doi.org/10.1038/nmeth.3179), BioID-class enzymes need no toxic peroxide step but label far more slowly; APEX2 labels within 30 s in the presence of hydrogen peroxide, yet showed no specific biotinylation when expressed at physiological knock-in levels, indicating a requirement for high copy numbers.[21](https://pubmed.ncbi.nlm.nih.gov/39056144/) Against classical affinity purification-MS, the two are complementary rather than alternatives: a four-bait comparison in trypanosomes found substantially distinct interaction sets, with affinity capture giving a snapshot of stable interactions at lysis and proximity labeling recording a history over the labeling period, biasing against stable core complexes in favor of dynamic associations.[22](https://pmc.ncbi.nlm.nih.gov/articles/PMC9791439/) At endogenous expression levels, 2 h of TurboID or miniTurbo labeling with 50 µM biotin outperformed 24 h of BioID2 labeling.[21](https://pubmed.ncbi.nlm.nih.gov/39056144/)\n\n## References\n\n1. [Kyle J. Roux and colleagues (2012). A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. The Journal of Cell Biology.](https://doi.org/10.1083/jcb.201112098)\n2. [Proximity-Dependent Biotinylation for Identification of Interacting Proteins (Current Protocols in Cell Biology, 2016)](https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpcb.11)\n3. [The development of proximity labeling technology and its applications in mammals, plants, and microorganisms (Cell Communication and Signaling, 2023)](https://link.springer.com/article/10.1186/s12964-023-01310-1)\n4. [Dae In Kim and colleagues (2014). Probing nuclear pore complex architecture with proximity-dependent biotinylation. Proceedings of the National Academy of Sciences.](https://doi.org/10.1073/pnas.1406459111)\n5. [Optimized Automated Workflow for BioID Improves Reproducibility and Identification of Protein–Protein Interactions (Mol Cell Proteomics, 2024)](https://www.sciencedirect.com/org/science/article/pii/S1535390724003597)\n6. [Tess C Branon and colleagues (2018). Efficient proximity labeling in living cells and organisms with TurboID. Nature Biotechnology.](https://doi.org/10.1038/nbt.4201)\n7. [Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches (review, 2020)](https://pmc.ncbi.nlm.nih.gov/articles/PMC7196579/)\n8. [Protocol: Defining the interactomes of proteins involved in cytoskeletal dynamics using high-throughput proximity-dependent biotinylation in cellulo (STAR Protocols)](https://www.sciencedirect.com/science/article/pii/S2666166721007814)\n9. [Spatial barcoding reveals reaction radii and contact-dependent mechanism of proximity labeling (Nature Chemical Biology 2025)](https://www.nature.com/articles/s41589-025-02086-w)\n10. [BioID: Identifying Protein-Protein Interactions in Living Cells (protocols.io working protocol)](https://www.protocols.io/view/bioid-identifying-protein-protein-interactions-in-dd8729zn.pdf)\n11. [Eunjoo Choi‐Rhee, Howard Schulman, John E. Cronan (2004). Promiscuous protein biotinylation by Escherichia coli biotin protein ligase. Protein Science.](https://doi.org/10.1110/ps.04911804)\n12. [Keehwan Kwon, Dorothy Beckett (2000). Function of a conserved sequence motif in biotin holoenzyme synthetases. Protein Science.](https://doi.org/10.1110/ps.9.8.1530)\n13. [Dae In Kim and colleagues (2016). An improved smaller biotin ligase for BioID proximity labeling. Molecular Biology of the Cell.](https://doi.org/10.1091/mbc.e15-12-0844)\n14. [Sofie De Munter and colleagues (2016). Split‐BioID: a proximity biotinylation assay for dimerization‐dependent protein interactions. FEBS Letters.](https://doi.org/10.1002/1873-3468.12548)\n15. [Isabel Myriam Schopp and colleagues (2017). Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nature Communications.](https://doi.org/10.1038/ncomms15690)\n16. [Kelvin F. Cho and colleagues (2020). Split-TurboID enables contact-dependent proximity labeling in cells. Proceedings of the National Academy of Sciences.](https://doi.org/10.1073/pnas.1919528117)\n17. [Kohki Kido and colleagues (2020). AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions. eLife.](https://doi.org/10.7554/elife.54983)\n18. [2C-BioID: An Advanced Two Component BioID System for Precision Mapping of Protein Interactomes (iScience, 2018)](https://doi.org/10.1016/j.isci.2018.11.023)\n19. [Coupling Proximity Biotinylation with Genomic Targeting to Characterize Locus-Specific Changes in Chromatin Environments (J Proteome Research, 2024/2025)](https://pubs.acs.org/jprobs/article-lookup/doi/10.1021/acs.jproteome.4c00931)\n20. [STUPPIT is a proximity labeling tool for labeling intermediary proteins that bridge two non-interacting proteins (PLOS Biology, 2025)](https://journals.plos.org/plosbiology/article?id=10.1371%2Fjournal.pbio.3003227)\n21. [When less is more - a fast TurboID knock-in approach for high-sensitivity endogenous interactome mapping (2024)](https://pubmed.ncbi.nlm.nih.gov/39056144/)\n22. [Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared](https://pmc.ncbi.nlm.nih.gov/articles/PMC9791439/)\n23. [Jeffrey D Martell and colleagues (2012). Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Nature Biotechnology.](https://doi.org/10.1038/nbt.2375)\n24. [Stephanie S Lam and colleagues (2014). Directed evolution of APEX2 for electron microscopy and proximity labeling. Nature Methods.](https://doi.org/10.1038/nmeth.3179)\n\n---\n*Topic: Encyclopedia › Life and health › Biological foundations › Biochemistry and metabolism › Biochemistry field and methods › Biochemical methods and techniques*\n\n*Initially written Sep 29, 2026 · Edited: Oct 15, 2026*\n\n*Copyright 2026 EdgeChat AI, a subsidiary of Biostate AI.*\n\nLicense: Edgepedia Community License 1.0, https://www.edgepedia.org/edgepedia/license\n", "same_as": [], "url": "https://www.edgepedia.org/bioid", "markdown_url": "https://www.edgepedia.org/bioid.md", "license": { "name": "Edgepedia Community License 1.0", "url": "https://www.edgepedia.org/edgepedia/license", "summary": "Free with credit, commercial use included. AI training is open to everyone. For other uses, organizations over USD 100M in revenue or 100M monthly users license separately.", "spdx": "LicenseRef-Edgepedia-Community-1.0" }, "credit": "\"BioID\", Edgepedia (EdgeChat), https://www.edgepedia.org/bioid. Edgepedia Community License 1.0.", "credit_md": "\"[BioID](https://www.edgepedia.org/bioid)\", Edgepedia (EdgeChat), [https://www.edgepedia.org/bioid](https://www.edgepedia.org/bioid). [Edgepedia Community License 1.0](https://www.edgepedia.org/edgepedia/license).", "credit_html": "\"BioID\", Edgepedia (EdgeChat), https://www.edgepedia.org/bioid. Edgepedia Community License 1.0.", "speakable": "BioID, or proximity-dependent biotin identification, is a method for mapping proteins near a chosen bait protein in living cells, introduced by Kyle J. Roux and colleagues in 2012." }