{ "id": "epdrbh2j4x", "slug": "capture-sequencing", "title": "Capture sequencing", "updated": "2026-10-15", "topic_path": [ { "id": "life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/topics/life" }, { "id": "life.biology", "label": "Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology" }, { "id": "life.biology.genetics", "label": "Genetics and genomic reference", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.genetics" }, { "id": "life.biology.genetics.genomics_projects_databases", "label": "Genomics, sequencing, and genome resources", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.genetics.genomics_projects_databases" }, { "id": "life.biology.genetics.genomics_projects_databases.targeted-sequencing-and-enrichment-methods", "label": "Targeted sequencing and enrichment methods", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.genetics.genomics_projects_databases.targeted-sequencing-and-enrichment-methods" } ], "geo": [ { "id": "geo.nongeo.t2001.life.biology.genetics.genomics_projects_databases", "label": "Non-geographic · 2001 to 2020: Genomics, sequencing, and genome resources", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.biology.genetics.genomics_projects_databases", "path": [ { "id": "geo.nongeo", "label": "Non-geographic", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo" }, { "id": "geo.nongeo.t2001", "label": "Non-geographic · 2001 to 2020", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001" }, { "id": "geo.nongeo.t2001.life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life" }, { "id": "geo.nongeo.t2001.life.biology", "label": "Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.biology" }, { "id": "geo.nongeo.t2001.life.biology.genetics", "label": "Genetics and genomic reference", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.biology.genetics" }, { "id": "geo.nongeo.t2001.life.biology.genetics.genomics_projects_databases", "label": "Genomics, sequencing, and genome resources", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.biology.genetics.genomics_projects_databases" } ] } ], "excerpt": "Capture sequencing is a targeted DNA sequencing method that uses biotinylated hybridization probes to enrich selected genomic regions, such as the exome or cancer gene panels, before sequencing.", "snippet": "Capture sequencing is a targeted DNA sequencing method that uses biotinylated hybridization probes to enrich selected genomic regions, such as the exome or cancer gene panels, before sequencing.", "node": "life.biology.genetics.genomics_projects_databases.targeted-sequencing-and-enrichment-methods", "markdown": "# Capture sequencing\n\nCapture sequencing is a targeted [DNA sequencing](https://www.edgepedia.org/dna-sequencing) method that enriches selected genomic regions with hybridization probes before sequencing. Instead of sequencing a whole genome at shallow depth, the laboratory isolates the fragments matching a chosen target set, such as the human exome or a cancer gene panel, and spends the sequencing budget only on those regions. Enrichment buys depth and efficiency: exome capture and sequencing costs roughly 10- to 20-fold less than whole-genome shotgun sequencing,[1](https://genomebiology.biomedcentral.com/counter/pdf/10.1186/gb-2011-12-1-r1.pdf)\n\n| Key fact | Value | Source |\n|---|---|---|\n| Core mechanism | Biotinylated DNA or RNA baits hybridize to target fragments; streptavidin beads pull down the bait-target complexes | [2](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture) |\n| First whole-exome solution capture | 36 Mb across >170,000 targets with 1.9 million probes (Bainbridge et al., 2010) | [3](https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-6-r62) |\n| Typical input DNA | 1-250 ng for library prep (IDT); as little as 20 ng with QIAseq xHYB dsDNA probes | [4](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing), [5](https://www.qiagen.com/ad/resources/download.aspx?id=773803c8-d61b-4270-83a1-2d824f48590b&lang=en) |\n| Typical hybridization | 2-48 h depending on panel and kit; xGen standard is 4 h at 65 °C, Twist is 16 h at 70 °C | [6](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/), [7](https://idtsfprod.blob.core.windows.net/sitefinity/docs/default-source/protocol/xgen-hybridization-capture-of-dna-libraries.pdf?sfvrsn=ab880a07_16), [8](https://www.twistbioscience.com/content/dam/twistbioscience/resources/2026-02/DOC-001273_Protocol_TargetEnrichment-StandardHyb-V2_REV6%20singles%20%281%29.pdf) |\n| Recommended depth | 50-500× for germline genotyping; >500× to 10,000× for somatic or rare-molecule detection | [9](https://sfvideo.blob.core.windows.net/sitefinity/docs/default-source/application-guide/idt-targeted-sequencing-guide.pdf?sfvrsn=ae151807_12) |\n| Uniformity benchmark | QIAseq xHYB Fold-80 penalty 1.3-1.5; best exome kits capture >94% of targets at 10× | [5](https://www.qiagen.com/ad/resources/download.aspx?id=773803c8-d61b-4270-83a1-2d824f48590b&lang=en), [10](https://eprints.soton.ac.uk/505280/2/lqaf115.pdf) |\n| Variant sensitivity | Down to 1% allele frequency without UMIs, versus 5% for amplicon sequencing | [4](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing) |\n\n## How it works\n\nThe method exploits nucleic acid hybridization. A sequencing library is first made by randomly shearing DNA and ligating sequencing adapters to the fragment ends, so captured fragments overlap and are unique rather than being fixed amplicon endpoints.[2](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture) Biotinylated oligonucleotide baits, typically 60-150 bases long, are then mixed with the denatured library and bind complementary target sequences[2](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture), [11](https://doi.org/10.1016/j.crmeth.2025.101174) Bait-target complexes are pulled out of solution with a magnet through the streptavidin-biotin bond.[2](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture)\n\nRNA baits hybridize to DNA targets with better specificity and duplex stability than DNA baits, because RNA:DNA hybrids are more stable than DNA:DNA hybrids; DNA probes nonetheless dominate commercially because RNA is chemically labile.[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/) Blocking reagents are essential: adapter-specific blockers occupy the ligated adapters and Cot DNA occupies repetitive elements, preventing nonspecific capture, and off-target capture itself arises from hybridization between repetitive genomic inserts and from adapter-mediated cross-hybridization[7](https://idtsfprod.blob.core.windows.net/sitefinity/docs/default-source/protocol/xgen-hybridization-capture-of-dna-libraries.pdf?sfvrsn=ab880a07_16), [12](https://link.springer.com/article/10.1186/s12864-025-11939-6) The importance of blocking is concrete: CATCH-Seq captures without Cot-1 DNA yielded less than 9% of mapped reads within target sites.[13](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0111756)\n\n## How it is done\n\nA representative solution-capture workflow runs as follows. The DNA is fragmented to the platform-recommended size, for example 150-350 bp in the IDT xGen protocol, and converted into an indexed library.[7](https://idtsfprod.blob.core.windows.net/sitefinity/docs/default-source/protocol/xgen-hybridization-capture-of-dna-libraries.pdf?sfvrsn=ab880a07_16) Libraries are pooled at equal mass for pre-capture multiplexing, with exome-scale multiplexing tested up to 12 samples (6 µg total DNA)[2](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture), [7](https://idtsfprod.blob.core.windows.net/sitefinity/docs/default-source/protocol/xgen-hybridization-capture-of-dna-libraries.pdf?sfvrsn=ab880a07_16) Blockers are added, the pool is denatured, and baits are hybridized; the xGen program is 95 °C for 30 seconds then 65 °C for 4 hours, extendable to 16 hours for GC-rich or small panels, while Twist uses 70 °C for 16 hours in a two-day workflow[7](https://idtsfprod.blob.core.windows.net/sitefinity/docs/default-source/protocol/xgen-hybridization-capture-of-dna-libraries.pdf?sfvrsn=ab880a07_16), [8](https://www.twistbioscience.com/content/dam/twistbioscience/resources/2026-02/DOC-001273_Protocol_TargetEnrichment-StandardHyb-V2_REV6%20singles%20%281%29.pdf) [Streptavidin](https://www.edgepedia.org/streptavidin) magnetic beads bind the baited complexes, wash buffers remove off-target DNA, and post-capture PCR amplifies the enriched pool before sequencing.[9](https://sfvideo.blob.core.windows.net/sitefinity/docs/default-source/application-guide/idt-targeted-sequencing-guide.pdf?sfvrsn=ae151807_12)\n\nTiming varies by kit. An oncology review reports hybridization of 2-48 h depending on target complexity, sample type, and DNA quality.[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/)\n\nPerformance is usually reported as on-target percentage, coverage uniformity (Fold-80 penalty), and required input. The original automated SHS protocol delivered 83.7% on-target bases, a Fold-80 penalty of 3.17, median coverage of 131.0×, and 4.4% duplicated reads across 1,117 libraries on 3 µg input.[1](https://genomebiology.biomedcentral.com/counter/pdf/10.1186/gb-2011-12-1-r1.pdf) Modern exome kits perform better: a systematic benchmark found Twist Custom Exome, Twist Human Comprehensive Exome, and KAPA HyperExome V1 captured >94% of targets at 10× with the lowest Fold-80 penalties.[10](https://eprints.soton.ac.uk/505280/2/lqaf115.pdf)\n\n## Origin\n\nSolution hybrid selection was reported by Andreas Gnirke and colleagues in [Nature Biotechnology](https://www.edgepedia.org/nature-biotechnology) in 2009; they used biotinylated RNA baits transcribed from microarray-synthesized oligodeoxynucleotides to fish targets out of a \"pond\" of DNA fragments, and with 170-mer baits targeting >15,000 exons plus four larger regions, about 90% of uniquely aligning bases fell on or near bait sequence.[14](https://doi.org/10.1038/nbt.1523) It built on earlier work: three 2007 papers described array-based capture, microarray direct selection by Thomas J. Albert and colleagues, microarray-based genomic selection by David T. Okou and colleagues, and genome-wide in situ exon capture by Emily Hodges and colleagues,[15](https://doi.org/10.1038/nmeth1111), [16](https://doi.org/10.1038/nmeth1109), [17](https://doi.org/10.1038/ng.2007.42) alongside multiplex exon amplification with molecular inversion probes by Gregory J. Porreca and colleagues.[18](https://doi.org/10.1038/nmeth1110) MIP capture itself traces to circularizing padlock probes introduced by [Mats Nilsson](https://www.edgepedia.org/mats-nilsson) and colleagues in 1994,[19](https://doi.org/10.1126/science.7522346) and BAC-based direct genomic selection by Stavros Bashiardes and colleagues in 2004 was a still earlier solution-capture precursor.[20](https://doi.org/10.1038/nmeth0105-63) Whole-exome demonstration followed quickly: Ng and colleagues sequenced 12 human exomes across more than 300 megabases of coding sequence in 2009,[21](https://www.nature.com/articles/nature08250) and Bainbridge and colleagues reported the first whole exome capture in solution in 2010.[3](https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-6-r62)\n\n## Variants\n\nExome capture targets the coding fraction of the genome; the Bainbridge design tiled 36 Mb of coding and miRNA targets with 1.9 million probes of 75 bp median length at 34 bp median spacing.[3](https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-6-r62) Published protocols and comparisons cover Agilent SureSelect, IDT xGen, Twist, Roche SeqCap and KAPA HyperExome, QIAseq xHYB, and myBaits, which differ mainly in bait chemistry (RNA versus DNA, single- versus double-stranded, synthesis method) and hybridization conditions[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/), [2](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture), [8](https://www.twistbioscience.com/content/dam/twistbioscience/resources/2026-02/DOC-001273_Protocol_TargetEnrichment-StandardHyb-V2_REV6%20singles%20%281%29.pdf), [5](https://www.qiagen.com/ad/resources/download.aspx?id=773803c8-d61b-4270-83a1-2d824f48590b&lang=en), [22](https://www.biocat.com/uploads/mybaits_custom_manual_v5.03_june.23-.pdf) The xGen Exome Research Panel v2 uses 415,115 individually synthesized oligonucleotide probes spanning a 34 Mb target region; QIAseq xHYB uses 5'-biotinylated double-stranded 120 nt DNA probes[4](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing), [5](https://www.qiagen.com/ad/resources/download.aspx?id=773803c8-d61b-4270-83a1-2d824f48590b&lang=en) Specialized variants include CATCH-Seq, which captures large contiguous 125 kb to 3.5 Mb blocks with RNA baits transcribed from sheared BAC clones,[13](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0111756) and GenCap-Seq, in which users make their own probes by shearing genomic DNA or amplicons to ~150 bp, biotinylating with terminal transferase, and denaturing to single strands.[23](https://journals.asm.org/doi/10.1128/mbio.01424-22)\n\nRecent variants move the bead-wash-PCR core, which had remained largely unchanged for over 15 years,[12](https://link.springer.com/article/10.1186/s12864-025-11939-6) in new directions. The Trinity approach captures baited libraries directly on a passivated streptavidin flow-cell surface, eliminating beads, washes, and post-capture PCR, and completes library prep to sequencer loading in under 5 hours.[12](https://link.springer.com/article/10.1186/s12864-025-11939-6) Targeted nanoEM couples capture with enzymatic base conversion for nanopore methylation analysis, reaching up to ×570 coverage at 5 kb N50 from 10 ng of input,[24](https://www.cell.com/cell-reports-methods/fulltext/S2667-2375%2825%2900251-6?uuid=uuid%3Ae185d276-2501-4a17-a5f1-6c969f20aed3) and scRaCH-seq applies capture to single-cell cDNA for long-read isoform and mutation detection.[25](https://genome.cshlp.org/content/early/2025/01/10/gr.279322.124.abstract)\n\n## Applications\n\nThe dominant clinical use is exome and panel sequencing for Mendelian disease: Ng and colleagues showed candidate genes for [Freeman-Sheldon syndrome](https://www.edgepedia.org/freeman-sheldon-syndrome) could be found from a small number of affected individuals,[21](https://www.nature.com/articles/nature08250) and Roca and colleagues diagnosed [congenital chloride diarrhea](https://www.edgepedia.org/congenital-chloride-diarrhea) by exome sequencing.[26](https://www.pnas.org/doi/10.1073/pnas.0910672106) In oncology, FDA-approved hybrid capture panels include FoundationOne CDx (324 cancer-related genes) and MSK-IMPACT (468 genes), which assess copy number changes, mutations, and structural rearrangements with TMB and MSI.[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/) Beyond human genetics, whole-genome in-solution capture was applied to ancient DNA with 6- to 159-fold enrichment across 12 ancient libraries,[27](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02924/pdf) and GenCap-Seq has been used to track intrastrain allele frequencies of [Pseudomonas aeruginosa](https://www.edgepedia.org/pseudomonas-aeruginosa) in cystic fibrosis sputum.[23](https://journals.asm.org/doi/10.1128/mbio.01424-22)\n\n## Limitations and alternatives\n\nHigh-GC regions reduce sensitivity for all enrichment chemistries,[28](https://pmc.ncbi.nlm.nih.gov/articles/PMC2945191/) and GC bias is method-dependent: in one comparison, HaloPlex and Nextera coverage peaked sharply near 60% GC while SureSelect and SeqCap performed consistently across GC content.[29](https://pmc.ncbi.nlm.nih.gov/articles/PMC4279426/) Other limitations include longer workflows, selection biases during capture and amplification, exclusion of non-targeted regions, and computational burdens of off-target read handling and deduplication; hybrid capture also carries elevated cost, high expertise requirements, and unsuitability for detecting completely novel microorganisms[11](https://doi.org/10.1016/j.crmeth.2025.101174), [27](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02924/pdf)\n\nAgainst alternatives, amplicon sequencing gives higher on-target rates but worse uniformity, and each amplicon method in one exome comparison missed variants detected by the other technologies, often through limited coverage or read-position effects.[30](https://pubmed.ncbi.nlm.nih.gov/26110913/) [Hybrid capture](https://www.edgepedia.org/hybrid-capture) methods outperformed amplicon approaches in library complexity, uniformity, and analytical sensitivity and specificity,[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/) and capture reaches panel sizes amplicons cannot.[4](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing) Compared with whole-genome sequencing, capture trades breadth for depth at roughly 10- to 20-fold lower cost for exomes.[1](https://genomebiology.biomedcentral.com/counter/pdf/10.1186/gb-2011-12-1-r1.pdf) Compared with sequencing-time alternatives, in a 2025 comparison ONT adaptive sampling achieved only about 2-3× enrichment versus 36.7× for the capture-based TEQUILA-seq, and the authors conclude capture should remain the method of choice when time and hands-on effort are not limiting.[31](https://link.springer.com/article/10.1186/s13059-025-03813-1) Hybridization temperature can be tuned to expected bait-target divergence for divergent or degraded samples: myBaits uses 65 °C below 10% divergence down to 60 °C for 15-25%.[22](https://www.biocat.com/uploads/mybaits_custom_manual_v5.03_june.23-.pdf)\n\n## References\n\n1. [Automated, highly scalable solution hybrid selection capture (Fisher et al., Genome Biology 2011)](https://genomebiology.biomedcentral.com/counter/pdf/10.1186/gb-2011-12-1-r1.pdf)\n2. [Targeted NGS by hybridization capture (IDT, merged with sgpages2.idtdna.com copy)](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing/hybridization-capture)\n3. [Whole exome capture in solution with 3 Gbp of data (Bainbridge et al., Genome Biology 2010)](https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-6-r62)\n4. [Targeted next generation sequencing (IDT, vendor technical resource)](https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing)\n5. [QIAseq xHYB Hybridization Capture handbook](https://www.qiagen.com/ad/resources/download.aspx?id=773803c8-d61b-4270-83a1-2d824f48590b&lang=en)\n6. [Target Enrichment Approaches for Next-Generation Sequencing Applications in Oncology (Journal of Molecular Diagnostics)](https://pmc.ncbi.nlm.nih.gov/articles/PMC9318977/)\n7. [IDT xGen hybridization capture of DNA libraries protocol (NGS-10122-PR)](https://idtsfprod.blob.core.windows.net/sitefinity/docs/default-source/protocol/xgen-hybridization-capture-of-dna-libraries.pdf?sfvrsn=ab880a07_16)\n8. [Twist Target Enrichment Standard protocol](https://www.twistbioscience.com/content/dam/twistbioscience/resources/2026-02/DOC-001273_Protocol_TargetEnrichment-StandardHyb-V2_REV6%20singles%20%281%29.pdf)\n9. [IDT Targeted Sequencing Guide Handbook (RUO22-0863)](https://sfvideo.blob.core.windows.net/sitefinity/docs/default-source/application-guide/idt-targeted-sequencing-guide.pdf?sfvrsn=ae151807_12)\n10. [A systematic analysis of contemporary whole exome sequencing capture kits to optimise high-coverage capture of CCDS regions](https://eprints.soton.ac.uk/505280/2/lqaf115.pdf)\n11. [Methods, applications, and computational challenges in bait capture enrichment (Cell Reports Methods, 2025)](https://doi.org/10.1016/j.crmeth.2025.101174)\n12. [A simplified hybrid capture approach retains high specificity and enables PCR-free workflow (Trinity, BMC Genomics 2025)](https://link.springer.com/article/10.1186/s12864-025-11939-6)\n13. [Targeted Sequencing of Large Genomic Regions with CATCH-Seq (PLOS One)](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0111756)\n14. [Andreas Gnirke and colleagues (2009). Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nature Biotechnology.](https://doi.org/10.1038/nbt.1523)\n15. [Thomas J Albert and colleagues (2007). Direct selection of human genomic loci by microarray hybridization. Nature Methods.](https://doi.org/10.1038/nmeth1111)\n16. [David T Okou and colleagues (2007). Microarray-based genomic selection for high-throughput resequencing. Nature Methods.](https://doi.org/10.1038/nmeth1109)\n17. [Emily Hodges and colleagues (2007). Genome-wide in situ exon capture for selective resequencing. Nature Genetics.](https://doi.org/10.1038/ng.2007.42)\n18. [Gregory J Porreca and colleagues (2007). Multiplex amplification of large sets of human exons. Nature Methods.](https://doi.org/10.1038/nmeth1110)\n19. [Mats Nilsson and colleagues (1994). Padlock Probes: Circularizing Oligonucleotides for Localized DNA Detection. Science.](https://doi.org/10.1126/science.7522346)\n20. [Stavros Bashiardes and colleagues (2004). Direct genomic selection. Nature Methods.](https://doi.org/10.1038/nmeth0105-63)\n21. [Targeted capture and massively parallel sequencing of 12 human exomes](https://www.nature.com/articles/nature08250)\n22. [myBaits Custom Hybridization Capture manual v5.03](https://www.biocat.com/uploads/mybaits_custom_manual_v5.03_june.23-.pdf)\n23. [Genome Capture Sequencing Selectively Enriches Bacterial DNA... (GenCap-Seq, mBio 2022)](https://journals.asm.org/doi/10.1128/mbio.01424-22)\n24. [S2667 2375(25)00251 6 (cell.com)](https://www.cell.com/cell-reports-methods/fulltext/S2667-2375%2825%2900251-6?uuid=uuid%3Ae185d276-2501-4a17-a5f1-6c969f20aed3)\n25. [Single-cell Rapid Capture Hybridization sequencing to reliably detect isoform usage and coding mutations in targeted genes (Genome Research, 2025)](https://genome.cshlp.org/content/early/2025/01/10/gr.279322.124.abstract)\n26. [Genetic diagnosis by whole exome capture and massively parallel DNA sequencing (PNAS)](https://www.pnas.org/doi/10.1073/pnas.0910672106)\n27. [Hybrid Capture-Based Next Generation Sequencing and Its Application to Human Infectious Diseases (Frontiers in Microbiology)](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02924/pdf)\n28. [Systematic comparison of three genomic enrichment methods for massively parallel DNA sequencing (Genome Research)](https://pmc.ncbi.nlm.nih.gov/articles/PMC2945191/)\n29. [Comparison of Custom Capture for Targeted Next-Generation DNA Sequencing](https://pmc.ncbi.nlm.nih.gov/articles/PMC4279426/)\n30. [Evaluation of Hybridization Capture Versus Amplicon-Based Methods for Whole-Exome Sequencing](https://pubmed.ncbi.nlm.nih.gov/26110913/)\n31. [Evaluating the potential and limitations of nanopore adaptive sampling for targeted transcriptome sequencing (Genome Biology 2025)](https://link.springer.com/article/10.1186/s13059-025-03813-1)\n\n---\n*Topic: Encyclopedia › Life and health › Biological foundations › Genetics and genomic reference › Genomics, sequencing, and genome resources › Targeted sequencing and enrichment methods*\n\n*Initially written Sep 29, 2026 · Reviewed: Sep 30, 2026 · Edited: Oct 15, 2026 · Last review: Sep 30, 2026*\n\n*Copyright 2026 EdgeChat AI, a subsidiary of Biostate AI.*\n\nLicense: Edgepedia Community License 1.0, https://www.edgepedia.org/edgepedia/license\n", "same_as": [], "url": "https://www.edgepedia.org/capture-sequencing", "markdown_url": "https://www.edgepedia.org/capture-sequencing.md", "license": { "name": "Edgepedia Community License 1.0", "url": "https://www.edgepedia.org/edgepedia/license", "summary": "Free with credit, commercial use included. AI training is open to everyone. For other uses, organizations over USD 100M in revenue or 100M monthly users license separately.", "spdx": "LicenseRef-Edgepedia-Community-1.0" }, "credit": "\"Capture sequencing\", Edgepedia (EdgeChat), https://www.edgepedia.org/capture-sequencing. Edgepedia Community License 1.0.", "credit_md": "\"[Capture sequencing](https://www.edgepedia.org/capture-sequencing)\", Edgepedia (EdgeChat), [https://www.edgepedia.org/capture-sequencing](https://www.edgepedia.org/capture-sequencing). [Edgepedia Community License 1.0](https://www.edgepedia.org/edgepedia/license).", "credit_html": "\"Capture sequencing\", Edgepedia (EdgeChat), https://www.edgepedia.org/capture-sequencing. Edgepedia Community License 1.0.", "speakable": "Capture sequencing is a targeted DNA sequencing method that uses biotinylated hybridization probes to enrich selected genomic regions, such as the exome or cancer gene panels, before sequencing." }