{ "id": "ep0mkz2nfx", "slug": "circulating-tumor-dna-analysis", "title": "Circulating tumor DNA analysis", "updated": "2026-10-15", "topic_path": [ { "id": "life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/topics/life" }, { "id": "life.health", "label": "Human health and medicine", "api_url": "https://www.edgepedia.org/api/v1/topics/life.health" }, { "id": "life.health.clinical", "label": "Clinical assessment and procedures", "api_url": "https://www.edgepedia.org/api/v1/topics/life.health.clinical" }, { "id": "life.health.clinical.clinical-diagnostics", "label": "Diagnosis and clinical assessment", "api_url": "https://www.edgepedia.org/api/v1/topics/life.health.clinical.clinical-diagnostics" }, { "id": "life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics", "label": "Laboratory and in-vitro diagnostics", "api_url": 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"Non-geographic · 1946 to 2000", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946" }, { "id": "geo.nongeo.t1946.life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life" }, { "id": "geo.nongeo.t1946.life.health", "label": "Human health and medicine", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.health" }, { "id": "geo.nongeo.t1946.life.health.clinical", "label": "Clinical assessment and procedures", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.health.clinical" }, { "id": "geo.nongeo.t1946.life.health.clinical.clinical-diagnostics", "label": "Diagnosis and clinical assessment", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.health.clinical.clinical-diagnostics" }, { "id": "geo.nongeo.t1946.life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics", "label": "Laboratory and in-vitro diagnostics", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics" } ] }, { "id": "geo.nongeo.t2001.life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics", "label": "Non-geographic · 2001 to 2020: Laboratory and in-vitro diagnostics", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics", "path": [ { "id": "geo.nongeo", "label": "Non-geographic", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo" }, { "id": "geo.nongeo.t2001", "label": "Non-geographic · 2001 to 2020", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001" }, { "id": "geo.nongeo.t2001.life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life" }, { "id": "geo.nongeo.t2001.life.health", "label": "Human health and medicine", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.health" }, { "id": "geo.nongeo.t2001.life.health.clinical", "label": "Clinical assessment and procedures", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.health.clinical" }, { "id": "geo.nongeo.t2001.life.health.clinical.clinical-diagnostics", "label": "Diagnosis and clinical assessment", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.health.clinical.clinical-diagnostics" }, { "id": "geo.nongeo.t2001.life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics", "label": "Laboratory and in-vitro diagnostics", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t2001.life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics" } ] } ], "excerpt": "Circulating tumor DNA analysis is a liquid biopsy method that detects tumor-derived DNA fragments in blood to guide cancer treatment, monitor recurrence, and screen for cancer.", "snippet": "Circulating tumor DNA analysis is a liquid biopsy method that detects tumor-derived DNA fragments in blood to guide cancer treatment, monitor recurrence, and screen for cancer.", "node": "life.health.clinical.clinical-diagnostics.laboratory-and-in-vitro-diagnostics.histopathology-and-tissue-based-diagnostics", "markdown": "# Circulating tumor DNA analysis\n\nCirculating tumor DNA (ctDNA) analysis is a liquid biopsy method that detects and quantifies tumor-derived DNA fragments in blood to guide treatment selection, minimal residual disease (MRD) detection, recurrence monitoring, and, more recently, cancer screening. The analyte is cell-free DNA (cfDNA) in plasma; the tumor-derived fraction usually appears at variant allele fractions (VAFs) below 1%.[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) Clinically, ctDNA testing is used to select patients with non-small cell lung cancer for EGFR-targeted therapy[2](https://www.annualreviews.org/content/journals/10.1146/annurev-med-041316-085721), and the FDA treats residual ctDNA after definitive local or (neo)adjuvant therapy as indicative of MRD usable for patient enrichment in early-stage trials.[3](https://www.fda.gov/media/183874/download)\n\n| Key fact | Value |\n|---|---|\n| ctDNA fragment size | ~120–180 bp, peak ~165 bp, consistent with apoptotic release[4](https://www.mdpi.com/1422-0067/26/2/861) |\n| Typical tumor fraction | VAF usually <1%; 1 mL plasma holds ~2,000 genome equivalents of cfDNA[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) |\n| Half-life | Reported as 90–120 min[5](https://arpi.unipi.it/retrieve/e0d6c931-58c2-fcf8-e053-d805fe0aa794/1-s2.0-S0009898121001893-main.pdf); other reviews give 16 min to 13 h[4](https://www.mdpi.com/1422-0067/26/2/861) |\n| Standard NGS sensitivity | Limited to VAFs of 2–5%; molecular barcoding and error suppression enable detection below 1%[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) |\n| ddPCR and BEAMing sensitivity | Analytical sensitivity of 0.01%–0.005%[4](https://www.mdpi.com/1422-0067/26/2/861) |\n| Detection by cancer type and stage | Detectable in >75% of advanced pancreatic, ovarian, colorectal, bladder, gastroesophageal, breast, melanoma, hepatocellular, and head and neck cancers, but <50% of primary brain, renal, prostate, or thyroid cancers[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC4017867/) |\n| Pre-analytics | Plasma preferred over serum; EDTA plasma separated within 4–6 h[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) |\n\n## How it works\n\nMost cfDNA is released by apoptotic cells. Fragments from normal cells span approximately 167 bp, the length of DNA wrapped around a nucleosome plus a linker; the most common cfDNA fragment is ~166 bp, and ctDNA tends to be shorter because its linker regions are trimmed.[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) • [7](https://www.gene-quantification.com/Johansson-Stahlberg-et-al-BDQ-SI-2019.pdf)\n\nTumor fraction tracks tumor burden and stage: ctDNA may comprise up to 10% of cfDNA in advanced disease, about 1% in locally advanced disease, and 0.1% in early-stage disease or after curative-intent treatment.[8](https://jitc.bmj.com/content/jitc/11/6/e006284.full.pdf) Because the half-life is short, measured at 90–120 min in one review[5](https://arpi.unipi.it/retrieve/e0d6c931-58c2-fcf8-e053-d805fe0aa794/1-s2.0-S0009898121001893-main.pdf) but 16 min to 13 h in another[4](https://www.mdpi.com/1422-0067/26/2/861), ctDNA levels respond rapidly to treatment, though published sources do not settle on a single range.\n\n## How it is done\n\nA typical draw collects at least 20 mL of whole blood.[9](https://www.jci.org/articles/view/154941) K2/K3-EDTA tubes are suitable but plasma should be separated within 4–6 hours by two-step centrifugation (800–1,600 × g, then 14,000–16,000 × g, 4 °C, 10 min each), with long-term storage at −80 °C in aliquots.[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) Cell-stabilizing tubes (Streck cfDNA BCT, PAXgene, Norgen, LBgard) prevent leukocyte lysis for several days at room temperature.[5](https://arpi.unipi.it/retrieve/e0d6c931-58c2-fcf8-e053-d805fe0aa794/1-s2.0-S0009898121001893-main.pdf) Serum is avoided because clotting lyses leukocytes and dilutes the tumor fraction.[10](https://link.springer.com/article/10.1186/s12943-022-01710-w)\n\nDetection technologies divide by breadth and sensitivity. PCR-based methods (qPCR, digital PCR, BEAMing) target known mutations; ddPCR partitions a 20 μL reaction into ~20,000 droplets read individually, and both ddPCR and BEAMing reach 0.01%–0.005% analytical sensitivity.[4](https://www.mdpi.com/1422-0067/26/2/861) Targeted NGS panels profile SNVs, indels, copy-number changes, and fusions comprehensively, but standard workflows are limited to VAFs of 2–5% unless molecular barcoding (UMIs) and in silico error suppression are applied.[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) Epigenomic assays add methylation and fragmentation signals: Shield combines fragmentomics, methylation, and somatic mutation callers into an integrated score with a limit of detection of 0.05% estimated tumor fraction[11](https://shieldcancerscreen.com/wp-content/uploads/LBL-000349_R1_Shield_Instructions_For_Use_1.pdf), and Guardant Reveal separates methylated from unmethylated molecules and preferentially enriches methylated tumor DNA.[12](https://www.guardantcomplete.com/assets/pdf/Methylation%20Whitepaper.pdf)\n\n## Origin\n\nMandel and Métais reported cell-free nucleic acids in human blood plasma in 1948, and Leon and colleagues showed in 1977 that free DNA was elevated in the serum of cancer patients[2](https://www.annualreviews.org/content/journals/10.1146/annurev-med-041316-085721); in 1994, mutant RAS gene fragments were found in the blood of cancer patients.[13](https://link.springer.com/article/10.1186/s13059-014-0449-4) Diehl and colleagues reported detection and quantification of mutations in the plasma of patients with colorectal tumors in 2005 in the [Proceedings of the National Academy of Sciences](https://www.edgepedia.org/proceedings-of-the-national-academy-of-sciences), finding a median of 47,800 APC fragments per mL of plasma (8% mutant) and mutant APC molecules in more than 60% of patients with early, presumably curable cancers, at 0.01%–1.7% of total APC molecules.[14](https://doi.org/10.1073/pnas.0507904102) The same group reported BEAMing, single-molecule PCR on microparticles in water-in-oil emulsions, in [Nature Methods](https://www.edgepedia.org/nature-methods) in 2006[15](https://doi.org/10.1038/nmeth898), and in 2008 quantified ctDNA in 162 plasma samples from 18 colorectal cancer patients in [Nature Medicine](https://www.edgepedia.org/nature-medicine), showing that postoperative ctDNA detection marked residual disease and recurrence risk.[16](https://doi.org/10.1038/nm.1789)\n\nSubsequent landmark reports include Murtaza and colleagues tracking acquired resistance by sequencing plasma DNA ([Nature](https://www.edgepedia.org/nature-journal), 2013)[17](https://doi.org/10.1038/nature12065); Bettegowda and colleagues detecting ctDNA across early- and late-stage malignancies ([Science Translational Medicine](https://www.edgepedia.org/science-translational-medicine), 2014)[6](https://pmc.ncbi.nlm.nih.gov/articles/PMC4017867/); Newman and colleagues describing CAPP-Seq, which detected ctDNA in 100% of stage II–IV NSCLC and 50% of stage I with 96% specificity down to ~0.02% VAF (Nature Medicine, 2014)[18](https://www.nature.com/articles/nm.3519); Tie and colleagues detecting MRD in stage II colon cancer (Science Translational Medicine, 2016)[19](https://doi.org/10.1126/scitranslmed.aaf6219); Phallen and colleagues reporting TEC-seq for early-stage detection (Science Translational Medicine, 2017)[20](https://doi.org/10.1126/scitranslmed.aan2415); the Merker-led ASCO and CAP joint review (Journal of Clinical Oncology, 2018)[21](https://doi.org/10.1200/jco.2017.76.8671); Parikh and colleagues reporting a plasma-only MRD assay in colorectal cancer (Clinical Cancer Research, 2021)[22](https://doi.org/10.1158/1078-0432.ccr-21-0410); and Tie and colleagues reporting the DYNAMIC trial (New England Journal of Medicine, 2022).[23](https://doi.org/10.1056/nejmoa2200075)\n\n## Variants\n\nThe FDA distinguishes tumor-informed assays, which sequence the tumor first and follow a selected variant set and may offer higher specificity, from tumor-naive (tumor-agnostic) panel-based NGS assays and smaller candidate-gene or multi-omics panels, each with its own strengths and limitations.[3](https://www.fda.gov/media/183874/download) Signatera CDx is a personalized, tumor-informed multiplex-PCR and NGS assay tracking 16 tumor-specific SNVs identified by whole-exome sequencing of FFPE tumor and matched whole blood, with germline and CHIP variants removed by subtraction; a sample is MRD-positive when at least two of the 16 are detected[24](https://www.accessdata.fda.gov/cdrh_docs/pdf26/P260004D.pdf), and a limit of detection of 0.004% has been described.[4](https://www.mdpi.com/1422-0067/26/2/861)\n\nTumor-naive panels profile plasma de novo: Guardant360 Liquid CDx detects SNVs and indels in 741 genes, copy-number amplifications in two genes, copy-number loss in one gene, and rearrangements in nine genes[25](https://www.accessdata.fda.gov/cdrh%5Fdocs/pdf25/P250027B.pdf), while FoundationOne Liquid CDx targets 324 genes from 20 ng extracted DNA.[4](https://www.mdpi.com/1422-0067/26/2/861)\n\n## Applications\n\nFor treatment selection, ctDNA testing is used to identify patients who may benefit from EGFR-targeted therapy in non-small cell lung cancer[2](https://www.annualreviews.org/content/journals/10.1146/annurev-med-041316-085721); the Roche cobas EGFR Mutation Test v2, the first FDA-approved ctDNA diagnostic, has an analytical limit of detection of approximately 5% VAF, reported to vary between 1.4% and 13.4% by mutation.[8](https://jitc.bmj.com/content/jitc/11/6/e006284.full.pdf)\n\nFor MRD and adjuvant decisions, the DYNAMIC trial showed that a ctDNA-guided strategy in stage II colon cancer reduced adjuvant chemotherapy use from 28% to 15% without compromising 2-year recurrence-free survival.[26](https://www.thelancet.com/journals/eclinm/article/PIIS2589-5370%2826%2900375-5/fulltext) For screening, the Shield blood test showed 83.1% sensitivity for colorectal cancer, 13.2% for advanced adenomas, and 89.6% specificity in the [ECLIPSE study](https://www.edgepedia.org/eclipse-study).[11](https://shieldcancerscreen.com/wp-content/uploads/LBL-000349_R1_Shield_Instructions_For_Use_1.pdf) ASCO approved its first guideline on ctDNA testing in solid tumors and lymphoma on November 18, 2025; it recommends ctDNA testing when tissue testing is challenging, delayed, or when a drug's indication allows it, recommends confirming negative results with tissue, and recommends against using fractional or concentration-based ctDNA measures as a surrogate of disease burden outside clinical trials.[27](https://ascopost.com/issues/july-25-2026/asco-publishes-first-guideline-on-ctdna-testing-in-solid-tumors-and-lymphoma)\n\n## Limitations and alternatives\n\nClonal hematopoiesis (CHIP), somatic mutations in DNA from normal blood cells, is the main false-positive source. CHIP affects genes including DNMT3A, TET2, and ASXL1, but also KRAS, TP53, and PIK3CA; paired PBMC sequencing and methylation-based tumor fraction reduce its impact.[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/) • [12](https://www.guardantcomplete.com/assets/pdf/Methylation%20Whitepaper.pdf)\n\nFalse negatives arise from low shedding and low VAF. A 2021 FDA evaluation found five commercial assays performed well above 0.5% VAF but were unreliable below it, with discordant results among vendors, labs, and replicates.[8](https://jitc.bmj.com/content/jitc/11/6/e006284.full.pdf) [Poisson sampling](https://www.edgepedia.org/poisson-sampling) constrains sensitivity: a 10 mL draw yielding ~5 mL plasma contains roughly 15 tumor DNA molecules at 0.1% VAF, and detecting one mutant molecule in 10,000 requires at least ~30 ng of DNA.[8](https://jitc.bmj.com/content/jitc/11/6/e006284.full.pdf) • [9](https://www.jci.org/articles/view/154941) The blood–brain barrier constrains tumor DNA release in CNS cancers and brain metastasis, where cerebrospinal fluid testing can be informative, and guidelines prefer reporting \"not detected\" or \"uninformative\" over \"negative\".[1](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/)\n\nCompared with tissue biopsy, ctDNA mutations are concordant up to 90% with matched tumors, with discrepancies mainly at low ctDNA levels[10](https://link.springer.com/article/10.1186/s12943-022-01710-w); a negative plasma result does not assure the tumor is negative, and patients should be reflexed to tissue testing when feasible.[25](https://www.accessdata.fda.gov/cdrh%5Fdocs/pdf25/P250027B.pdf) Absence of ctDNA cannot currently be equated with cure.[9](https://www.jci.org/articles/view/154941) The 2018 ASCO/CAP review pointed to limited evidence of clinical validity and utility for most assays outside trials.[10](https://link.springer.com/article/10.1186/s12943-022-01710-w) • [21](https://doi.org/10.1200/jco.2017.76.8671)\n\n## References\n\n1. [Clinical Practice Guideline for Blood-based Circulating Tumor DNA Assays](https://pmc.ncbi.nlm.nih.gov/articles/PMC10813828/)\n2. [Circulating Tumor DNA: Measurement and Clinical Utility](https://www.annualreviews.org/content/journals/10.1146/annurev-med-041316-085721)\n3. [FDA Guidance for Industry: Use of ctDNA as a Biomarker in Early-Stage Solid Tumor Drug Development](https://www.fda.gov/media/183874/download)\n4. [Detection of Circulating Tumor DNA in Liquid Biopsy: Current Techniques and Potential Applications in Melanoma (IJMS, 2025)](https://www.mdpi.com/1422-0067/26/2/861)\n5. [What do we need to obtain high quality circulating tumor DNA (ctDNA) for routine diagnostic test in oncology? – Considerations on pre-analytical aspects by the IFCC workgroup cfDNA](https://arpi.unipi.it/retrieve/e0d6c931-58c2-fcf8-e053-d805fe0aa794/1-s2.0-S0009898121001893-main.pdf)\n6. [Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies (Bettegowda et al.)](https://pmc.ncbi.nlm.nih.gov/articles/PMC4017867/)\n7. [Considerations and quality controls when analyzing cell-free tumor DNA (Biomolecular Detection and Quantification, 2019)](https://www.gene-quantification.com/Johansson-Stahlberg-et-al-BDQ-SI-2019.pdf)\n8. [Genomic approaches to cancer and minimal residual disease detection using circulating tumor DNA](https://jitc.bmj.com/content/jitc/11/6/e006284.full.pdf)\n9. [Circulating tumor DNA: current challenges for clinical utility](https://www.jci.org/articles/view/154941)\n10. [Circulating tumor nucleic acids: biology, release mechanisms, and clinical relevance](https://link.springer.com/article/10.1186/s12943-022-01710-w)\n11. [Shield Colorectal Cancer Screening Test Instructions for Use](https://shieldcancerscreen.com/wp-content/uploads/LBL-000349_R1_Shield_Instructions_For_Use_1.pdf)\n12. [Guardant Reveal: Quantifying ctDNA Using a Tissue-Free Test for MRD Detection (Guardant Health whitepaper)](https://www.guardantcomplete.com/assets/pdf/Methylation%20Whitepaper.pdf)\n13. [Genotyping cell-free tumor DNA in the blood to detect residual disease and drug resistance](https://link.springer.com/article/10.1186/s13059-014-0449-4)\n14. [Frank Diehl and colleagues (2005). Detection and quantification of mutations in the plasma of patients with colorectal tumors. Proceedings of the National Academy of Sciences.](https://doi.org/10.1073/pnas.0507904102)\n15. [Frank Diehl and colleagues (2006). BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Nature Methods.](https://doi.org/10.1038/nmeth898)\n16. [Frank Diehl and colleagues (2008). Circulating mutant DNA to assess tumor dynamics. Nature Medicine.](https://doi.org/10.1038/nm.1789)\n17. [Muhammed Murtaza and colleagues (2013). Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA. Nature.](https://doi.org/10.1038/nature12065)\n18. [An ultrasensitive method for quantitating circulating tumor DNA with broad patient coverage (CAPP-Seq)](https://www.nature.com/articles/nm.3519)\n19. [Jeanne Tie and colleagues (2016). Circulating tumor DNA analysis detects minimal residual disease and predicts recurrence in patients with stage II colon cancer. Science Translational Medicine.](https://doi.org/10.1126/scitranslmed.aaf6219)\n20. [Jillian Phallen and colleagues (2017). Direct detection of early-stage cancers using circulating tumor DNA. Science Translational Medicine.](https://doi.org/10.1126/scitranslmed.aan2415)\n21. [Jason D. Merker and colleagues (2018). Circulating Tumor DNA Analysis in Patients With Cancer: American Society of Clinical Oncology and College of American Pathologists Joint Review. Journal of Clinical Oncology.](https://doi.org/10.1200/jco.2017.76.8671)\n22. [Aparna R. Parikh and colleagues (2021). Minimal Residual Disease Detection using a Plasma-only Circulating Tumor DNA Assay in Patients with Colorectal Cancer. Clinical Cancer Research.](https://doi.org/10.1158/1078-0432.ccr-21-0410)\n23. [Jeanne Tie and colleagues (2022). Circulating Tumor DNA Analysis Guiding Adjuvant Therapy in Stage II Colon Cancer. New England Journal of Medicine.](https://doi.org/10.1056/nejmoa2200075)\n24. [Signatera CDx PMA summary (Natera)](https://www.accessdata.fda.gov/cdrh_docs/pdf26/P260004D.pdf)\n25. [Guardant360 Liquid CDx (P250027) Summary of Safety and Effectiveness](https://www.accessdata.fda.gov/cdrh%5Fdocs/pdf25/P250027B.pdf)\n26. [Circulating tumor DNA in gastrointestinal cancers: promise, pitfalls, and the path forward (eClinicalMedicine)](https://www.thelancet.com/journals/eclinm/article/PIIS2589-5370%2826%2900375-5/fulltext)\n27. [ASCO Publishes First Guideline on ctDNA Testing in Solid Tumors and Lymphoma (The ASCO Post)](https://ascopost.com/issues/july-25-2026/asco-publishes-first-guideline-on-ctdna-testing-in-solid-tumors-and-lymphoma)\n\n---\n*Topic: Encyclopedia › Life and health › Human health and medicine › Clinical assessment and procedures › Diagnosis and clinical assessment › Laboratory and in-vitro diagnostics › Histopathology and tissue-based diagnostics*\n\n*Initially written Sep 29, 2026 · Reviewed: Sep 30, 2026 · Edited: Oct 15, 2026 · Last review: Sep 30, 2026*\n\n*Copyright 2026 EdgeChat AI, a subsidiary of Biostate AI.*\n\nLicense: Edgepedia Community License 1.0, https://www.edgepedia.org/edgepedia/license\n", "same_as": [], "url": "https://www.edgepedia.org/circulating-tumor-dna-analysis", "markdown_url": "https://www.edgepedia.org/circulating-tumor-dna-analysis.md", "license": { "name": "Edgepedia Community License 1.0", "url": "https://www.edgepedia.org/edgepedia/license", "summary": "Free with credit, commercial use included. AI training is open to everyone. For other uses, organizations over USD 100M in revenue or 100M monthly users license separately.", "spdx": "LicenseRef-Edgepedia-Community-1.0" }, "credit": "\"Circulating tumor DNA analysis\", Edgepedia (EdgeChat), https://www.edgepedia.org/circulating-tumor-dna-analysis. Edgepedia Community License 1.0.", "credit_md": "\"[Circulating tumor DNA analysis](https://www.edgepedia.org/circulating-tumor-dna-analysis)\", Edgepedia (EdgeChat), [https://www.edgepedia.org/circulating-tumor-dna-analysis](https://www.edgepedia.org/circulating-tumor-dna-analysis). [Edgepedia Community License 1.0](https://www.edgepedia.org/edgepedia/license).", "credit_html": "\"Circulating tumor DNA analysis\", Edgepedia (EdgeChat), https://www.edgepedia.org/circulating-tumor-dna-analysis. Edgepedia Community License 1.0.", "speakable": "Circulating tumor DNA analysis is a liquid biopsy method that detects tumor-derived DNA fragments in blood to guide cancer treatment, monitor recurrence, and screen for cancer." }