{ "id": "ep2gq8cj2w", "slug": "protein-footprinting", "title": "Protein footprinting", "updated": "2026-10-15", "topic_path": [ { "id": "life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/topics/life" }, { "id": "life.biology", "label": "Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology" }, { "id": "life.biology.biochemistry", "label": "Biochemistry and metabolism", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.biochemistry" }, { "id": "life.biology.biochemistry.practice", "label": "Biochemistry field and methods", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.biochemistry.practice" }, { "id": "life.biology.biochemistry.practice.methods", "label": "Biochemical methods and techniques", "api_url": "https://www.edgepedia.org/api/v1/topics/life.biology.biochemistry.practice.methods" } ], "geo": [ { "id": "geo.nongeo.t1946.life.biology.biochemistry.practice.methods", "label": "Non-geographic · 1946 to 2000: Biochemical methods and techniques", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.biology.biochemistry.practice.methods", "path": [ { "id": "geo.nongeo", "label": "Non-geographic", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo" }, { "id": "geo.nongeo.t1946", "label": "Non-geographic · 1946 to 2000", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946" }, { "id": "geo.nongeo.t1946.life", "label": "Life and health", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life" }, { "id": "geo.nongeo.t1946.life.biology", "label": "Biological foundations", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.biology" }, { "id": "geo.nongeo.t1946.life.biology.biochemistry", "label": "Biochemistry and metabolism", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.biology.biochemistry" }, { "id": "geo.nongeo.t1946.life.biology.biochemistry.practice", "label": "Biochemistry field and methods", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.biology.biochemistry.practice" }, { "id": "geo.nongeo.t1946.life.biology.biochemistry.practice.methods", "label": "Biochemical methods and techniques", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.life.biology.biochemistry.practice.methods" } ] } ], "excerpt": "Protein footprinting is a solution-phase structural biology method that uses chemical or radical probes and mass spectrometry to map protein interaction surfaces, binding sites, and conformational change.", "snippet": "Protein footprinting is a solution-phase structural biology method that uses chemical or radical probes and mass spectrometry to map protein interaction surfaces, binding sites, and conformational change.", "node": "life.biology.biochemistry.practice.methods", "markdown": "# Protein footprinting\n\nProtein footprinting is a solution-phase structural biology method that uses chemical modification or cleavage probes to map the solvent accessibility of amino acid side chains and backbone amides, revealing protein–protein interaction surfaces, ligand-binding sites, folding, and conformational change.[1](https://www.nature.com/articles/s41592-026-03083-0) Quantitative changes in labeling between two states report on solvent accessibility and reveal effects of protein–protein interactions, ligand binding, protein folding, conformational changes, or applied stress.[1](https://www.nature.com/articles/s41592-026-03083-0) In a differential experiment comparing a ligand-bound and unbound state, differences in uptake reflect changes in backbone protection, including hydrogen bonding and solvent exposure, and matched workflows can reduce, but not eliminate, back-exchange effects.[2](https://pubs.acs.org/doi/full/10.1021/acs.accounts.4c00545)\n\n| Key fact | Detail |\n|---|---|\n| What is measured | Solvent accessibility of side chains (radical methods) or backbone amides (HDX), read out by LC–MS[1](https://www.nature.com/articles/s41592-026-03083-0) |\n| Three labeling classes | Reversible HDX, slow specific side-chain labeling, fast irreversible radical labeling on the submillisecond timescale[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/) |\n| FPOP chemistry | 248 nm KrF excimer laser photolyzes 15 mM H₂O₂; with 20 mM glutamine scavenger, radical lifetimes are ~1 μs[4](https://pubs.acs.org/doi/full/10.1021/acs.chemrev.1c00432) |\n| Side-chain coverage | Hydroxyl radicals react with 14 of 20 side chains in FPOP[5](https://www.nature.com/articles/s41596-020-0396-3); labeling efficiency varies about 1,000-fold across residues[6](https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-070317-033123) |\n| HDX-MS resolution | Typically 5–20 residues, set by proteolytic peptide length[7](https://pmc.ncbi.nlm.nih.gov/articles/PMC11570944/) |\n| Term coined | Sheshberadaran and Payne, 1988, for limited proteolysis of antibody-bound antigen[8](https://doi.org/10.1073/pnas.85.1.1) |\n| Key failure modes | Back exchange (~30%, sometimes ≥50% in HDX), radical scavenging by Tris/HEPES buffers, over- and under-oxidation[7](https://pmc.ncbi.nlm.nih.gov/articles/PMC11570944/) • [9](https://www.mdpi.com/2073-4468/11/4/71) |\n\n## How it works\n\nFootprinting approaches divide into three classes by probe and speed.[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/) Reversible hydrogen–deuterium exchange (HDX) replaces backbone amide hydrogens with deuterium from D₂O, whose effective concentration exceeds 50 M, so exchange follows pseudo-first-order kinetics and covers all amino acids except proline; the footprint reflects solvent-accessible surface area and hydrogen bonding, but the labeling is reversible.[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/) Slow, irreversible labeling modifies specific side chains with reagents such as carbodiimides. Fast, irreversible radical labeling uses highly reactive species that modify several side-chain types broadly on the submillisecond timescale.[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/)\n\nHydroxyl radicals are generated from hydrogen peroxide by photolysis, Fenton chemistry, or electrochemistry, or directly from water by X-rays, plasma, or gamma rays; the covalent oxidation products are read out by standard LC–MS workflows.[1](https://www.nature.com/articles/s41592-026-03083-0) Hydroxyl radicals can label over 70% of the side chains in a typical protein, but labeling efficiency varies about 1,000-fold across the 20 residues, from highly reactive methionine and cysteine to essentially unreactive alanine and glycine.[6](https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-070317-033123) Because fast reactions proceed faster than protein folding or unfolding, the chemistry captures the structure without biasing it; slow footprinting needs no special apparatus such as a laser or synchrotron.[2](https://pubs.acs.org/doi/full/10.1021/acs.accounts.4c00545)\n\n## How it is done\n\nIn bottom-up radical footprinting, a footprinted protein is digested by a protease, and the resulting peptides are separated, detected, and sequenced by LC–MS/MS, with the fraction of modification quantified as the footprint.[5](https://www.nature.com/articles/s41596-020-0396-3) In FPOP, a flow system with an exclusion volume between laser shots ensures each sample bolus sees a single pulse, and a scavenger such as glutamine or histidine reduces the hydroxyl radical lifetime to microseconds, minimizing overlabeling.[5](https://www.nature.com/articles/s41596-020-0396-3) Dose–response plots of the fraction unmodified against exposure time reveal which residues become more or less solvent accessible; the fraction modified is typically several percent, except for highly reactive residues such as methionine.[9](https://www.mdpi.com/2073-4468/11/4/71) Protection factor (PF) analysis corrects observed rate constants for intrinsic residue chemical reactivity, enabling absolute topology measurements.[6](https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-070317-033123) Data-processing software includes PEAKS Studio, Proteome Discoverer, XCalibur, Mass Spec Studio, Mascot, Protein Metrics suite, and Protein Prospector.[5](https://www.nature.com/articles/s41596-020-0396-3)\n\nThe standard HDX-MS workflow labels with D₂O at near-neutral pH around 295 K, quenches by acidification to pH 2.5 at approximately 0 °C, digests with pepsin, and runs reverse-phase LC with online ESI-MS, keeping post-quench steps under 20 min; commercial systems digest at around 15 °C to boost peptide yield.[7](https://pmc.ncbi.nlm.nih.gov/articles/PMC11570944/) Because low-energy collisional activation induces deuterium scrambling, electron-based fragmentation (ECD or ETD) is required for residue-level deuterium uptake.[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/)\n\n## Origin\n\nThe term \"footprint\" for solvent protection was in use by the 1970s, when chemical or enzymatic digests mapped the protective footprint of a protein on DNA.[9](https://www.mdpi.com/2073-4468/11/4/71) Tullius and Dombroski introduced hydroxyl radical \"footprinting\" of DNA–protein contacts in 1986.[10](https://doi.org/10.1073/pnas.83.15.5469) Sheshberadaran and Payne coined the term \"protein footprinting\" in 1988 for limited proteolysis of monoclonal-antibody-bound antigen to locate contact sites.[8](https://doi.org/10.1073/pnas.85.1.1) Smith, Deng, and Zhang reported amide hydrogen exchange with mass spectrometry for protein structure in 1997.[11](https://doi.org/10.1002/%28sici%291096-9888%28199702%2932:2<135::aid-jms486>3.0.co;2-m) Radical probe mass spectrometry (RP-MS) with hydroxyl radicals generated directly from water was introduced in 1998 at the ASMS annual conference and first published in 1999 by Maleknia, Brenowitz, and Chance using synchrotron X-ray radiolysis.[12](https://europepmc.org/article/MED/30484400) • [13](https://doi.org/10.1021/ac990500e) [Carbodiimide](https://www.edgepedia.org/carbodiimide) coupling had earlier been applied to proteins in 1966 to quantify Asp and Glu residues, later becoming the basis of GEE footprinting.[14](https://par.nsf.gov/servlets/purl/10688308) Hambly and Gross introduced FPOP in 2005 by laser flash photolysis of hydrogen peroxide on the microsecond timescale.[15](https://doi.org/10.1016/j.jasms.2005.09.008)\n\n## Variants\n\n**HDX-MS** labels reversible backbone amides and reports on SASA and hydrogen bonding.[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/) **Synchrotron radiolysis footprinting** generates hydroxyl radicals in millisecond pulses of a synchrotron white beam; solvent-accessible cysteine, methionine, phenylalanine, tyrosine, tryptophan, histidine, proline, and leucine serve as probes, covering around 30% of a typical protein's sequence.[16](https://commons.case.edu/cgi/viewcontent.cgi?article=1209&context=facultyworks) **FPOP** photolyzes H₂O₂ with a 248 nm excimer laser on the microsecond timescale.[4](https://pubs.acs.org/doi/full/10.1021/acs.chemrev.1c00432) Related photochemical approaches include photochemical oxidation surface mapping by Sharp, Becker, and Hettich (2003)[17](https://doi.org/10.1021/ac0302004) and nanosecond laser-induced photochemical oxidation by Aye, Low, and Sze (2005).[18](https://doi.org/10.1021/ac050353m) Watson and colleagues reported pulsed electron beam water radiolysis for submicrosecond footprinting in 2009.[19](https://doi.org/10.1021/ac802252y) The laser-free Flash Oxidation (FOX) platform, reported by Sharp and colleagues in 2021, extends FPOP-style footprinting without a laser.[20](https://doi.org/10.1021/jasms.0c00471) The FPOP platform also accommodates carbenes, carbonate radical anion, and photochemical iodination, and supports in vivo labeling of live C. elegans.[3](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/) [Carbene](https://www.edgepedia.org/carbene) footprinting on the FPOP platform was reported by Zhang, Rempel, and Gross in 2015.[21](https://doi.org/10.1007/s13361-015-1313-9) Laser-initiated radical trifluoromethylation adds a fluorine-tagged probe, reported by Cheng and colleagues in 2017.[22](https://doi.org/10.1002/anie.201706697) Carboxyl-group footprinting uses glycine ethyl ester (GEE) with EDC, or the more stable glycine propargyl amide (GPA), whose adducts do not hydrolyze under normal proteomic-pipeline conditions.[14](https://par.nsf.gov/servlets/purl/10688308) SPROX (stability of proteins from rates of oxidation), a chemical-oxidation stability assay, and DEPC labeling, a covalent modification of nucleophilic side chains, are related oxidation or labeling methods rather than hydroxyl-radical variants.[14](https://par.nsf.gov/servlets/purl/10688308)\n\n## Applications\n\nFPOP has been applied to epitope mapping, protein aggregation, small-molecule binding-site location, ligand-binding affinity measurement, folding and unfolding monitoring, and hidden conformational changes.[5](https://www.nature.com/articles/s41596-020-0396-3) Jones and colleagues reported FPOP for epitope mapping in 2011.[23](https://doi.org/10.1021/ac2007366) [Footprinting](https://www.edgepedia.org/footprinting) is applied to biotherapeutics, metal-bound proteins, aggregating amyloid proteins, and integral membrane proteins, cases where high-resolution structural methods struggle.[2](https://pubs.acs.org/doi/full/10.1021/acs.accounts.4c00545) Espino, Mali, and Jones reported in-cell footprinting coupled with mass spectrometry (IC-FPOP) for structural analysis of proteins in live cells in 2015;[24](https://doi.org/10.1021/acs.analchem.5b01888) catalase breaking down H₂O₂ to produce O₂ makes FPOP viable for in vivo footprinting in whole live cells and intact C. elegans.[9](https://www.mdpi.com/2073-4468/11/4/71) A consensus Perspective in [Nature Methods](https://www.edgepedia.org/nature-methods) now provides a framework for experimental design, sample processing, data analysis, interpretation, and integration with orthogonal data in academic and biopharmaceutical research.[1](https://www.nature.com/articles/s41592-026-03083-0) Over the past decade, oxidative footprinting has proven valuable as a solution-phase and in-cell method, with recent innovations extending HRPF to cell lysates and intact cells and increasing throughput.[1](https://www.nature.com/articles/s41592-026-03083-0) • [4](https://pubs.acs.org/doi/full/10.1021/acs.chemrev.1c00432)\n\n## Limitations and alternatives\n\nIn HDX-MS, solution and gas-phase back exchange cause peptides to lose a substantial fraction of backbone deuteration, often around 30% but sometimes 50% or more, and the widely used back-exchange correction can distort kinetic profiles when back exchange is 30% or higher and exchange rates are heterogeneous.[7](https://pmc.ncbi.nlm.nih.gov/articles/PMC11570944/) HDX's reversibility constrains analysis for membrane and glycosylated proteins, whereas fast radical footprinting is irreversible with submillisecond labeling.[5](https://www.nature.com/articles/s41596-020-0396-3) For radical methods, common buffers such as Tris and HEPES scavenge hydroxyl radicals efficiently, so HRPF is usually run in phosphate or N-cacodylate buffer.[9](https://www.mdpi.com/2073-4468/11/4/71) Too little oxidation leaves less-reactive residues unmodified, while too much may alter the protein's structure, so dosimetry with internal and external standards tunes the experiment; stable secondary radicals can persist beyond the millisecond timescale and require quenching.[6](https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-070317-033123)\n\nHRPF is a low-resolution method that does not fully describe a protein system and is coupled with orthogonal methods such as HDX (backbone dynamics), SAXS, ion mobility spectrometry, and cryo-EM.[4](https://pubs.acs.org/doi/full/10.1021/acs.chemrev.1c00432) HDX probes reversible backbone accessibility and can measure a wider range of conformational changes, while HRF irreversibly probes side-chain accessibility.[6](https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-070317-033123) A systematic comparison of four solution-based structural proteomics MS approaches, hydrogen exchange, chemical cross-linking, limited proteolysis, and radical probe footprinting, made the case to preference radical probe footprinting when all facets are considered side by side.[25](https://www.sciencedirect.com/science/article/abs/pii/S0165993618304424) No single footprint is sufficient, and complementary approaches are needed for structure comparisons.[2](https://pubs.acs.org/doi/full/10.1021/acs.accounts.4c00545) Footprinting data combined with computational modeling can test and refine structural models of macromolecular complexes,[26](https://www.annualreviews.org/content/journals/10.1146/annurev.biophys.35.040405.102050) and docking algorithms such as PROXIMO, reported by Gerega and Downard in 2006, model complexes from radical probe MS data.[27](https://doi.org/10.1093/bioinformatics/btl178) Despite this, HRPF has not achieved the industrial adoption of HDX, in part because LC–MS is not seamlessly integrated into commercial footprinting systems.[9](https://www.mdpi.com/2073-4468/11/4/71)\n\n## References\n\n1. [Recommendations and considerations for hydroxyl radical protein footprinting–mass spectrometry](https://www.nature.com/articles/s41592-026-03083-0)\n2. [Mass Spectrometry-Based Protein Footprinting for Protein Structure Characterization (Accounts of Chemical Research)](https://pubs.acs.org/doi/full/10.1021/acs.accounts.4c00545)\n3. [Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications (Chem. Rev. 2020; Europe PMC abstract and university PDF copy merged)](https://pmc.ncbi.nlm.nih.gov/articles/PMC7531764/)\n4. [Hydroxyl Radical Protein Footprinting: A Mass Spectrometry-Based Structural Method for Studying the Higher Order Structure of Proteins (Chemical Reviews)](https://pubs.acs.org/doi/full/10.1021/acs.chemrev.1c00432)\n5. [Protein higher-order-structure determination by fast photochemical oxidation of proteins and mass spectrometry analysis (Nature Protocols)](https://www.nature.com/articles/s41596-020-0396-3)\n6. [High-Resolution Hydroxyl Radical Protein Footprinting: Biophysics Tool for Drug Discovery (Annual Review of Biophysics)](https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-070317-033123)\n7. [Hydrogen/Deuterium Exchange Mass Spectrometry: Fundamentals, Limitations, and Opportunities](https://pmc.ncbi.nlm.nih.gov/articles/PMC11570944/)\n8. [H Sheshberadaran, L G Payne (1988). Protein antigen-monoclonal antibody contact sites investigated by limited proteolysis of monoclonal antibody-bound antigen: protein \"footprinting\".. Proceedings of the National Academy of Sciences.](https://doi.org/10.1073/pnas.85.1.1)\n9. [Structural Investigation of Therapeutic Antibodies Using Hydroxyl Radical Protein Footprinting Methods (MDPI Antibodies; PMC copy merged)](https://www.mdpi.com/2073-4468/11/4/71)\n10. [T D Tullius, B A Dombroski (1986). Hydroxyl radical \"footprinting\": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein.. Proceedings of the National Academy of Sciences.](https://doi.org/10.1073/pnas.83.15.5469)\n11. [Probing the Non-covalent Structure of Proteins by Amide Hydrogen Exchange and Mass Spectrometry (Journal of Mass Spectrometry, 1997)](https://doi.org/10.1002/%28sici%291096-9888%28199702%2932:2<135::aid-jms486>3.0.co;2-m)\n12. [Protein Footprinting with Radical Probe Mass Spectrometry - Two Decades of Achievement (Protein and Peptide Letters, 2019)](https://europepmc.org/article/MED/30484400)\n13. [Simin D. Maleknia, Michael Brenowitz, Mark R. Chance (1999). Millisecond Radiolytic Modification of Peptides by Synchrotron X-rays Identified by Mass Spectrometry. Analytical Chemistry.](https://doi.org/10.1021/ac990500e)\n14. [J. Am. Soc. Mass Spectrom. 2024.35:3192-3202 (GPA click-enrichable carboxyl-group footprinting)](https://par.nsf.gov/servlets/purl/10688308)\n15. [David M. Hambly, Michael L. Gross (2005). Laser flash photolysis of hydrogen peroxide to oxidize protein solvent-accessible residues on the microsecond timescale. Journal of the American Society for Mass Spectrometry.](https://doi.org/10.1016/j.jasms.2005.09.008)\n16. [Structural Analysis of Gelsolin Using Synchrotron Protein Footprinting (Molecular & Cellular Proteomics)](https://commons.case.edu/cgi/viewcontent.cgi?article=1209&context=facultyworks)\n17. [Joshua S. Sharp, Jeffrey M. Becker, Robert L. Hettich (2003). Analysis of Protein Solvent Accessible Surfaces by Photochemical Oxidation and Mass Spectrometry. Analytical Chemistry.](https://doi.org/10.1021/ac0302004)\n18. [Thin Thin Aye, Teck Yew Low, Siu Kwan Sze (2005). Nanosecond Laser-Induced Photochemical Oxidation Method for Protein Surface Mapping with Mass Spectrometry. Analytical Chemistry.](https://doi.org/10.1021/ac050353m)\n19. [Caroline Watson and colleagues (2009). Pulsed Electron Beam Water Radiolysis for Submicrosecond Hydroxyl Radical Protein Footprinting. Analytical Chemistry.](https://doi.org/10.1021/ac802252y)\n20. [Joshua S. Sharp and colleagues (2021). Flash Oxidation (FOX) System: A Novel Laser-Free Fast Photochemical Oxidation Protein Footprinting Platform. Journal of the American Society for Mass Spectrometry.](https://doi.org/10.1021/jasms.0c00471)\n21. [Bojie Zhang, Don L. Rempel, Michael L. Gross (2015). Protein Footprinting by Carbenes on a Fast Photochemical Oxidation of Proteins (FPOP) Platform. Journal of the American Society for Mass Spectrometry.](https://doi.org/10.1007/s13361-015-1313-9)\n22. [Ming Cheng and colleagues (2017). Laser‐Initiated Radical Trifluoromethylation of Peptides and Proteins: Application to Mass‐Spectrometry‐Based Protein Footprinting. Angewandte Chemie International Edition.](https://doi.org/10.1002/anie.201706697)\n23. [Lisa M. Jones and colleagues (2011). Fast Photochemical Oxidation of Proteins for Epitope Mapping. Analytical Chemistry.](https://doi.org/10.1021/ac2007366)\n24. [Jessica A. Espino, Vishaal S. Mali, Lisa M. Jones (2015). In Cell Footprinting Coupled with Mass Spectrometry for the Structural Analysis of Proteins in Live Cells. Analytical Chemistry.](https://doi.org/10.1021/acs.analchem.5b01888)\n25. [Mass spectrometry in structural proteomics: The case for radical probe protein footprinting (TrAC Trends in Analytical Chemistry)](https://www.sciencedirect.com/science/article/abs/pii/S0165993618304424)\n26. [Radiolytic Protein Footprinting with Mass Spectrometry to Probe the Structure of Macromolecular Complexes (Annual Review of Biophysics, 2006)](https://www.annualreviews.org/content/journals/10.1146/annurev.biophys.35.040405.102050)\n27. [Sebastien K. Gerega, Kevin M. Downard (2006). PROXIMO, a new docking algorithm to model protein complexes using data from radical probe mass spectrometry (RP-MS). Bioinformatics.](https://doi.org/10.1093/bioinformatics/btl178)\n\n---\n*Topic: Encyclopedia › Life and health › Biological foundations › Biochemistry and metabolism › Biochemistry field and methods › Biochemical methods and techniques*\n\n*Initially written Sep 29, 2026 · Edited: Oct 15, 2026*\n\n*Copyright 2026 EdgeChat AI, a subsidiary of Biostate AI.*\n\nLicense: Edgepedia Community License 1.0, https://www.edgepedia.org/edgepedia/license\n", "same_as": [], "url": "https://www.edgepedia.org/protein-footprinting", "markdown_url": "https://www.edgepedia.org/protein-footprinting.md", "license": { "name": "Edgepedia Community License 1.0", "url": "https://www.edgepedia.org/edgepedia/license", "summary": "Free with credit, commercial use included. AI training is open to everyone. For other uses, organizations over USD 100M in revenue or 100M monthly users license separately.", "spdx": "LicenseRef-Edgepedia-Community-1.0" }, "credit": "\"Protein footprinting\", Edgepedia (EdgeChat), https://www.edgepedia.org/protein-footprinting. Edgepedia Community License 1.0.", "credit_md": "\"[Protein footprinting](https://www.edgepedia.org/protein-footprinting)\", Edgepedia (EdgeChat), [https://www.edgepedia.org/protein-footprinting](https://www.edgepedia.org/protein-footprinting). [Edgepedia Community License 1.0](https://www.edgepedia.org/edgepedia/license).", "credit_html": "\"Protein footprinting\", Edgepedia (EdgeChat), https://www.edgepedia.org/protein-footprinting. Edgepedia Community License 1.0.", "speakable": "Protein footprinting is a solution-phase structural biology method that uses chemical or radical probes and mass spectrometry to map protein interaction surfaces, binding sites, and conformational change." }