{ "id": "epv1009488", "slug": "stopped-flow-spectroscopy", "title": "Stopped-flow spectroscopy", "updated": "2026-10-21", "topic_path": [ { "id": "physical", "label": "Physical world and mathematics", "api_url": "https://www.edgepedia.org/api/v1/topics/physical" }, { "id": "physical.chemistry", "label": "Chemistry", "api_url": "https://www.edgepedia.org/api/v1/topics/physical.chemistry" }, { "id": "physical.chemistry.methods", "label": "Chemical principles and methods", "api_url": "https://www.edgepedia.org/api/v1/topics/physical.chemistry.methods" }, { "id": "physical.chemistry.methods.chemical-reaction-science", "label": "Reaction rates, mechanisms, and engineering", "api_url": "https://www.edgepedia.org/api/v1/topics/physical.chemistry.methods.chemical-reaction-science" }, { "id": "physical.chemistry.methods.chemical-kinetics-and-reaction-engineering", "label": "Chemical kinetics and reaction engineering", "api_url": "https://www.edgepedia.org/api/v1/topics/physical.chemistry.methods.chemical-kinetics-and-reaction-engineering" } ], "geo": [ { "id": "geo.nongeo.t1946.physical.chemistry.methods.chemical-reaction-science", "label": "Non-geographic · 1946 to 2000: Reaction rates, mechanisms, and engineering", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.physical.chemistry.methods.chemical-reaction-science", "path": [ { "id": "geo.nongeo", "label": "Non-geographic", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo" }, { "id": "geo.nongeo.t1946", "label": "Non-geographic · 1946 to 2000", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946" }, { "id": "geo.nongeo.t1946.physical", "label": "Physical world and mathematics", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.physical" }, { "id": "geo.nongeo.t1946.physical.chemistry", "label": "Chemistry", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.physical.chemistry" }, { "id": "geo.nongeo.t1946.physical.chemistry.methods", "label": "Chemical principles and methods", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.physical.chemistry.methods" }, { "id": "geo.nongeo.t1946.physical.chemistry.methods.chemical-reaction-science", "label": "Reaction rates, mechanisms, and engineering", "api_url": "https://www.edgepedia.org/api/v1/geo/geo.nongeo.t1946.physical.chemistry.methods.chemical-reaction-science" } ] } ], "excerpt": "Stopped-flow spectroscopy is a rapid-mixing technique in which reactants are mixed, the flow is abruptly stopped, and the reaction is followed spectroscopically in a fixed cell.", "snippet": "Stopped-flow spectroscopy is a rapid-mixing technique in which reactants are mixed, the flow is abruptly stopped, and the reaction is followed spectroscopically in a fixed cell.", "node": "physical.chemistry.methods.chemical-kinetics-and-reaction-engineering", "markdown": "# Stopped-flow spectroscopy\n\nStopped-flow spectroscopy is a rapid-mixing technique in which two solutions are driven into a mixer, the flow is abruptly arrested, and the resulting reaction is followed spectroscopically in a fixed observation cell. It is the most common means of studying fast solution-phase reactions, reaching time intervals down to a fraction of a millisecond while using far less solution than continuous-flow methods.[1](https://chem.libretexts.org/Courses/University_of_Wisconsin_Oshkosh/Chem_370%3A_Physical_Chemistry_1_-_Thermodynamics_%28Gutow%29/04%3A_Reaction_Kinetics/4.02%3A_Measuring_Reaction_Rates) The effective dead time of a typical instrument is 1 to 2 ms, so reactions with half times shorter than this cannot be studied,[2](https://uhra.herts.ac.uk/id/eprint/328/1/902016.pdf) although individual instruments range from about 1 to 10 ms depending on design.[3](https://www.sciencedirect.com/science/article/abs/pii/S0076687918303896) The method occupies the gap between manual mixing, generally more reliable for reactions with time constants longer than a few minutes,[4](https://link.springer.com/rwe/10.1007/978-3-642-16712-6_59) and relaxation or photolysis methods that reach microsecond and faster timescales.\n\n| Key fact | Detail |\n|---|---|\n| What it measures | Absorbance, fluorescence, or related optical signals from a reaction initiated by mixing, converted to concentration or fitted as rate constants |\n| Timescale | Roughly 1 ms to minutes; dead time typically 1–2 ms, best commercial designs 0.5–0.85 ms[2](https://uhra.herts.ac.uk/id/eprint/328/1/902016.pdf) • [5](https://www.photophysics.com/media/e1ncgk1t/stopped-flow-brochure-updated-app_digital.pdf) • [6](https://kintekcorp.com/products/sf-300x/) |\n| Trigger | A microswitch in the backstop of the stop syringe plunger starts data collection[4](https://link.springer.com/rwe/10.1007/978-3-642-16712-6_59) |\n| Sample use | About 20 µL per reactant per shot on modern instruments; 120 µL on accessory-type units[6](https://kintekcorp.com/products/sf-300x/) • [7](https://www.photophysics.com/media/5pfjyjia/rx2000-product-information.pdf) |\n| Precursor | Continuous-flow method of Hartridge and Roughton (1923), which consumed large volumes[8](https://doi.org/10.1098/rspa.1923.0116) |\n| Main variants | Quench-flow, sequential (double) mixing, cryogenic and high-pressure operation, temperature-jump combinations |\n| Detection modes | UV-Vis absorbance and fluorescence as standard; CD, NMR, EPR, conductivity, and SAXS as specialized couplings |\n\n## How it works\n\nTwo drive syringes push reactants through a small mixing chamber into an observation cell, where the detector is placed at a fixed point close to the mixer. A third stopping syringe opposes the flow; when its plunger hits a backstop fitted with a microswitch, flow stops abruptly and data collection begins.[4](https://link.springer.com/rwe/10.1007/978-3-642-16712-6_59) In a common arrangement the reactants travel separately to the cuvette and mix only on entering the cell, while previously reacted solution is ejected into a waste syringe whose plunger trips the switch.[9](https://www.agilent.com/cs/library/applications/uv75.pdf) Because observation happens after the flow has stopped, stopped-flow analysis generally does not require converting position or flow velocity into reaction time, which is why it has been adopted so widely; the dead time and mixing performance must still be characterized for the instrument and conditions used.[10](https://pubmed.ncbi.nlm.nih.gov/16328835/)\n\nThe dead time is the time for the freshly mixed plug to travel from the mixer to the observation point, a few milliseconds in conventional instruments, plus the time to arrest the flow.[11](http://www.sfu.ca/~brodovit/files/chem366/manual/06_STOfl131.pdf) Mixing itself takes a couple of milliseconds and must be much shorter than the reaction lifetime.[11](http://www.sfu.ca/~brodovit/files/chem366/manual/06_STOfl131.pdf) [Absorbance](https://www.edgepedia.org/absorbance) traces are converted to concentration through the Beer-Lambert law, where \\( \\epsilon \\) is the molar absorptivity and \\( \\ell \\) the path length,[1](https://chem.libretexts.org/Courses/University_of_Wisconsin_Oshkosh/Chem_370%3A_Physical_Chemistry_1_-_Thermodynamics_%28Gutow%29/04%3A_Reaction_Kinetics/4.02%3A_Measuring_Reaction_Rates) and the time course is fitted to sums of exponentials to extract observed rate constants \\( k_{\\mathrm{obs}} \\).\n\n## How it is done\n\nThe practitioner loads the drive syringes, primes the lines, and sets flow rate and temperature. Two checks precede real experiments: mixing efficiency is tested with a pH-indicator proton-transfer reaction such as 4-methylumbelliferone in pyrophosphate buffer, and dead time is measured with the N-acetyltryptophanamide (NAT) plus N-bromosuccinimide (NBS) fluorescence-quenching reaction, which has a known half time of about 20 ms.[2](https://uhra.herts.ac.uk/id/eprint/328/1/902016.pdf)\n\nReplication and fitting complete the workflow. Traces from 5 to 6 shots of the same reaction are averaged before analysis, and the instrument dead time sets the first usable data point.[12](https://josephgroup.ucsd.edu/Protocols/Protocols%20PDF/Guidelines%20Analyzing%20StoppedFlow.pdf) Linear sampling suits time courses under 30 s, while logarithmic sampling is preferred when fast and slow phases coexist;[12](https://josephgroup.ucsd.edu/Protocols/Protocols%20PDF/Guidelines%20Analyzing%20StoppedFlow.pdf) a logarithmic timebase for this purpose was introduced by Adrian R. Walmsley and Clive R. Bagshaw in 1989.[13](https://doi.org/10.1016/0003-2697%2889%2990315-1) The analysis window is chosen by the six-half-life rule, since a reaction is 98% complete over six half-lives, with \\( t_{1/2} = 0.693/k_{\\mathrm{obs}} \\).[12](https://josephgroup.ucsd.edu/Protocols/Protocols%20PDF/Guidelines%20Analyzing%20StoppedFlow.pdf) Traces are fitted by nonlinear regression, for example Levenberg-Marquardt, with quality judged by residuals, standard errors, and confidence intervals.[12](https://josephgroup.ucsd.edu/Protocols/Protocols%20PDF/Guidelines%20Analyzing%20StoppedFlow.pdf)\n\n## Origin\n\nThe precursor was the continuous-flow method that [Hamilton Hartridge](https://www.edgepedia.org/hamilton-hartridge) and [Francis John Worsley Roughton](https://www.edgepedia.org/francis-john-worsley-roughton) published in Proceedings of the Royal Society A on 1 October 1923 for measuring the velocity of very rapid chemical reactions.[8](https://doi.org/10.1098/rspa.1923.0116) Continuous flow required large sample volumes, and stopped flow was developed in the 1940s by modifying it, with the principal advantage of more economic use of reagents.[4](https://link.springer.com/rwe/10.1007/978-3-642-16712-6_59) [Britton Chance](https://www.edgepedia.org/britton-chance) applied rapid flow methods to the peroxidase–hydrogen peroxide enzyme-substrate compound in the Journal of Biological Chemistry in 1943,[14](https://doi.org/10.1016/s0021-9258%2818%2944929-0) and published the accelerated and stopped-flow methods in Review of Scientific Instruments in 1951.[15](https://pubs.aip.org/aip/rsi/article/22/8/619/297792/Rapid-and-Sensitive-Spectrophotometry-I-The) That apparatus gave satisfactory records with only a few tenths of a cubic centimeter of 2×10⁻⁷ M iron enzyme solution.[15](https://pubs.aip.org/aip/rsi/article/22/8/619/297792/Rapid-and-Sensitive-Spectrophotometry-I-The)\n\n[Quentin Gibson](https://www.edgepedia.org/quentin-gibson) described a stopped-flow apparatus of his own design in Discussions of the Faraday Society in 1954,[16](https://doi.org/10.1039/df9541700137) and Gibson and Milnes published a widely adopted apparatus design in the Biochemical Journal in 1964.[17](https://doi.org/10.1042/bj0910161) A commercial Gibson-Durrum apparatus appeared in the early 1960s and was widely distributed.[18](https://www.jstage.jst.go.jp/article/analsci1985/4/5/4_5_445/_pdf)\n\n## Variants\n\n**Quench-flow** halts the reaction after a set interval by chemical quenching, freeze quenching, or optical quenching, and the mixture is then analyzed off-line.[1](https://chem.libretexts.org/Courses/University_of_Wisconsin_Oshkosh/Chem_370%3A_Physical_Chemistry_1_-_Thermodynamics_%28Gutow%29/04%3A_Reaction_Kinetics/4.02%3A_Measuring_Reaction_Rates) A pulsed quenched-flow technique was applied by [Alan R. Fersht](https://www.edgepedia.org/alan-r-fersht) and Ross Jakes in 1975 to demonstrate two reaction pathways for aminoacylation of tRNA.[19](https://doi.org/10.1021/bi00686a010) **Sequential or double mixing** mixes two reactants, ages the mixture for a pre-selected time, then mixes with a third solution; the KinTek SF-300X uses a delay line for pH-jump and multi-step enzymatic studies.[6](https://kintekcorp.com/products/sf-300x/) **Cryogenic operation** extends standard -20 to +85 °C ranges down to -90 °C with liquid-nitrogen cooling.[20](https://www.biologic.net/wp-content/uploads/2019/08/stopped-flow-in-cryogenic-conditions_sfm-2000-series_rapid-kinetics-an25.pdf) **High-pressure stopped flow** operates from -40 to +100 °C and up to 200 MPa, with a dead time below 2 ms that is pressure independent, enabling activation-volume measurements.[21](https://pubmed.ncbi.nlm.nih.gov/21619372/) Combining stopped flow with fast-scan spectroscopy or a [temperature jump](https://www.edgepedia.org/temperature-jump) helps identify transient intermediates.[22](https://www.chem.tamu.edu/rgroup/marcetta/chem636/Presentations/Stopped%20Flow%20Analysis.pdf)\n\nDetection spans UV/Vis, IR, fluorescence, chemiluminescence, circular dichroism, NMR, EPR, voltammetry, and conductivity.[22](https://www.chem.tamu.edu/rgroup/marcetta/chem636/Presentations/Stopped%20Flow%20Analysis.pdf) [Fluorescence](https://www.edgepedia.org/fluorescence) and absorbance are the principal commercial modes. Stopped-flow CD is limited to roughly the 10 ms range by poor signal-to-noise and flow artifacts such as strain-induced birefringence.[2](https://uhra.herts.ac.uk/id/eprint/328/1/902016.pdf) Stopped-flow NMR gives data points within 2 to 10 s, limited mainly by the spin-lattice relaxation time \\( T_1 \\).[22](https://www.chem.tamu.edu/rgroup/marcetta/chem636/Presentations/Stopped%20Flow%20Analysis.pdf) Stopped-flow has also been coupled to fluorescence lifetime and to small-angle [X-ray scattering](https://www.edgepedia.org/x-ray-scattering) (SAXS); at the SSRL BL 4-2 beamline a four-syringe SFM-400 with over 0.25 ms dead time gives 5 ms detector-limited SAXS time resolution,[23](https://www-ssrl.slac.stanford.edu/~saxs/download/weiss_tr.pdf) and at CoSAXS stopped-flow SAXS reaches 2 ms timescales with good signal-to-noise.[24](https://researchprofiles.ku.dk/en/publications/time-resolved-scattering-methods-for-biological-samples-at-the-co/)\n\n## Applications\n\nThe method was created for enzyme kinetics: Chance's peroxidase work measured a bimolecular rate constant of 10⁷ M⁻¹ s⁻¹ with micromolar enzyme and a 1 ms rise time.[10](https://pubmed.ncbi.nlm.nih.gov/16328835/) In protein binding, stopped-flow determines association and dissociation rate constants for intrinsically disordered protein partners, distinguishes induced-fit from conformational-selection mechanisms, and supports Φ-value analysis of the binding transition state.[3](https://www.sciencedirect.com/science/article/abs/pii/S0076687918303896) An application to analytical chemistry was differential kinetic analysis of metal–CyDTA ion-exchange reactions at 10⁻⁴ to 10⁻⁵ M.[18](https://www.jstage.jst.go.jp/article/analsci1985/4/5/4_5_445/_pdf) Stopped-flow SAXS has resolved structural kinetics, following compaction of acid-denatured cytochrome c during refolding through changes in radius of gyration.[25](https://inano.au.dk/fileadmin/inano/iNANOSchool/iNANOschool_2007/iNANOschool%20_2008/N9/Otzen%20article%202%20Roder%20rapid%20kinetics%20review.pdf)\n\n## Limitations and alternatives\n\nDead times are difficult to push below about 1 ms because of the time delay and artifacts caused by abruptly arresting the flow.[26](https://mdpi-res.com/d_attachment/molecules/molecules-27-03392/article_deploy/molecules-27-03392.pdf?version=1653461619) The ultimate limit on rapid-mixing time resolution is cavitation: at high flow velocities, pressure gradients across turbulent eddies create solvent vapor bubbles that scatter light and can make kinetic detection virtually impossible.[26](https://mdpi-res.com/d_attachment/molecules/molecules-27-03392/article_deploy/molecules-27-03392.pdf?version=1653461619) At the slow end, reactions with half times longer than about 10 s can be complicated by lamp instabilities and photobleaching,[2](https://uhra.herts.ac.uk/id/eprint/328/1/902016.pdf) and for time constants beyond a few minutes manual mixing is generally more reliable.[4](https://link.springer.com/rwe/10.1007/978-3-642-16712-6_59)\n\nFor reactions complete in less than a millisecond, the alternatives are [relaxation methods](https://www.edgepedia.org/relaxation-method), which perturb an existing equilibrium with rapid pressure or temperature jumps, or flash photolysis for photolabile systems; in temperature-jump relaxation of a first-order reversible reaction the relaxation rate is \\( k_1 + k_{-1} \\), so a plot of \\( \\ln|\\Delta x| \\) versus time has slope \\( -(k_1 + k_{-1}) \\).[27](https://chem.libretexts.org/Courses/Pacific_Union_College/Kinetics/08%3A_Chemical_Kinetics/8.10%3A_Fast_Reactions_in_Solution) Relaxation methods such as laser-induced temperature jump monitor shorter timescales but require different fitting equations.[3](https://www.sciencedirect.com/science/article/abs/pii/S0076687918303896) [Flash photolysis](https://www.edgepedia.org/flash-photolysis) uses a short-duration flash lamp, work for which they shared the 1967 [Nobel Prize in Chemistry](https://www.edgepedia.org/nobel-prize-in-chemistry).[27](https://chem.libretexts.org/Courses/Pacific_Union_College/Kinetics/08%3A_Chemical_Kinetics/8.10%3A_Fast_Reactions_in_Solution) Continuous-flow microfluidic mixing reaches far shorter dead times, 3.8 ± 0.3 µs in one instrument with a 2.7 µs mixing time,[28](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0185888) and microfabricated versions reach 12 to 27 µs depending on viscosity,[26](https://mdpi-res.com/d_attachment/molecules/molecules-27-03392/article_deploy/molecules-27-03392.pdf?version=1653461619) but continuous flow consumes substantially larger sample volumes, typically at millimolar concentrations for sufficient absorbance over ~100 µm paths.[3](https://www.sciencedirect.com/science/article/abs/pii/S0076687918303896) • [28](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0185888)\n\n## References\n\n1. [4.02: Measuring Reaction Rates (chem.libretexts.org)](https://chem.libretexts.org/Courses/University_of_Wisconsin_Oshkosh/Chem_370%3A_Physical_Chemistry_1_-_Thermodynamics_%28Gutow%29/04%3A_Reaction_Kinetics/4.02%3A_Measuring_Reaction_Rates)\n2. [Rapid Kinetic Techniques (Eccleston, Hutchinson, White chapter; repository copy)](https://uhra.herts.ac.uk/id/eprint/328/1/902016.pdf)\n3. [Stopped-Flow Kinetic Techniques for Studying Binding Reactions of Intrinsically Disordered Proteins (Methods in Enzymology)](https://www.sciencedirect.com/science/article/abs/pii/S0076687918303896)\n4. [Stopped-Flow Techniques (Bagshaw, Encyclopedia of Biophysics, 2013)](https://link.springer.com/rwe/10.1007/978-3-642-16712-6_59)\n5. [SX Series of Stopped-Flow Spectrometers (Applied Photophysics brochure)](https://www.photophysics.com/media/e1ncgk1t/stopped-flow-brochure-updated-app_digital.pdf)\n6. [SF-300X stopped-flow instrument (KinTek Corporation)](https://kintekcorp.com/products/sf-300x/)\n7. [RX2000 Rapid Mixing Stopped-Flow Accessory (Applied Photophysics)](https://www.photophysics.com/media/5pfjyjia/rx2000-product-information.pdf)\n8. [Hamilton Hartridge, Francis John Worsley Roughton (1923). A method of measuring the velocity of very rapid chemical reactions. Proceedings of the Royal Society of London Series A Containing Papers of a Mathematical and Physical Character.](https://doi.org/10.1098/rspa.1923.0116)\n9. [Monitoring Fast Chemical Reactions Using Stopped Flow Kinetics (Agilent application note, 2021)](https://www.agilent.com/cs/library/applications/uv75.pdf)\n10. [The Stopped-flow Method and Chemical Intermediates in Enzyme Reactions - A Personal Essay (Chance 2004; full text at life.illinois.edu/history/Chance2004.pdf)](https://pubmed.ncbi.nlm.nih.gov/16328835/)\n11. [Stop Flow Kinetics (SFU Chem 366 lab manual)](http://www.sfu.ca/~brodovit/files/chem366/manual/06_STOfl131.pdf)\n12. [Guidelines for Acquiring and Analyzing Stopped-Flow Data (Simpson Joseph, 2008)](https://josephgroup.ucsd.edu/Protocols/Protocols%20PDF/Guidelines%20Analyzing%20StoppedFlow.pdf)\n13. [Logarithmic timebase for stopped-flow data acquisition and analysis (Analytical Biochemistry, 1989)](https://doi.org/10.1016/0003-2697%2889%2990315-1)\n14. [THE KINETICS OF THE ENZYME-SUBSTRATE COMPOUND OF PEROXIDASE (Journal of Biological Chemistry, 1943)](https://doi.org/10.1016/s0021-9258%2818%2944929-0)\n15. [Rapid and Sensitive Spectrophotometry. I. The Accelerated and Stopped-Flow Methods...](https://pubs.aip.org/aip/rsi/article/22/8/619/297792/Rapid-and-Sensitive-Spectrophotometry-I-The)\n16. [Q. H. Gibson (1954). Stopped-flow apparatus for the study of rapid reactions. Discussions of the Faraday Society.](https://doi.org/10.1039/df9541700137)\n17. [QH Gibson, L Milnes (1964). Apparatus for rapid and sensitive spectrophotometry. Biochemical Journal.](https://doi.org/10.1042/bj0910161)\n18. [Stopped-flow time difference analysis (SFTDA), Analytical Sciences 1988](https://www.jstage.jst.go.jp/article/analsci1985/4/5/4_5_445/_pdf)\n19. [Alan R. Fersht, Ross Jakes (1975). Demonstration of two reaction pathways for the aminoacylation of tRNA. Application of the pulsed quenched flow technique. Biochemistry.](https://doi.org/10.1021/bi00686a010)\n20. [AN25: Stopped-flow in cryogenic configuration (Bio-Logic)](https://www.biologic.net/wp-content/uploads/2019/08/stopped-flow-in-cryogenic-conditions_sfm-2000-series_rapid-kinetics-an25.pdf)\n21. [High-pressure stopped-flow spectrometer for kinetic studies of fast reactions by absorbance and fluorescence detection (Anal Chem 1996)](https://pubmed.ncbi.nlm.nih.gov/21619372/)\n22. [Stopped Flow Techniques for Measuring Kinetics (Yeung, TAMU CHEM636 seminar)](https://www.chem.tamu.edu/rgroup/marcetta/chem636/Presentations/Stopped%20Flow%20Analysis.pdf)\n23. [Time-resolved SAXS short introduction (T.M. Weiss, SSRL/SLAC)](https://www-ssrl.slac.stanford.edu/~saxs/download/weiss_tr.pdf)\n24. [Time-resolved scattering methods for biological samples at the CoSAXS beamline, MAX IV Laboratory (Methods in Enzymology vol. 709, 2024)](https://researchprofiles.ku.dk/en/publications/time-resolved-scattering-methods-for-biological-samples-at-the-co/)\n25. [Rapid kinetics review (Röder; Methods, doi:10.1016/j.ymeth.2004.03.003)](https://inano.au.dk/fileadmin/inano/iNANOSchool/iNANOschool_2007/iNANOschool%20_2008/N9/Otzen%20article%202%20Roder%20rapid%20kinetics%20review.pdf)\n26. [Advances in Mixer Design and Detection Methods for Kinetics Studies ... on the Microsecond Time Scale (Molecules 2022, 27, 3392)](https://mdpi-res.com/d_attachment/molecules/molecules-27-03392/article_deploy/molecules-27-03392.pdf?version=1653461619)\n27. [8.10: Fast Reactions in Solution (Chemistry LibreTexts)](https://chem.libretexts.org/Courses/Pacific_Union_College/Kinetics/08%3A_Chemical_Kinetics/8.10%3A_Fast_Reactions_in_Solution)\n28. [Microsecond time-scale kinetics of transient biochemical reactions (PLOS One)](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0185888)\n\n---\n*Topic: Encyclopedia › Physical world and mathematics › Chemistry › Chemical principles and methods › Reaction rates, mechanisms, and engineering › Chemical kinetics and reaction engineering*\n\n*Initially written Sep 29, 2026 · Edited: Oct 15, 2026; Oct 21, 2026*\n\n*Copyright 2026 EdgeChat AI, a subsidiary of Biostate AI.*\n\nLicense: Edgepedia Community License 1.0, https://www.edgepedia.org/edgepedia/license\n", "same_as": [ "https://www-ssrl.slac.stanford.edu/~saxs/download/weiss_tr.pdf" ], "url": "https://www.edgepedia.org/stopped-flow-spectroscopy", "markdown_url": "https://www.edgepedia.org/stopped-flow-spectroscopy.md", "license": { "name": "Edgepedia Community License 1.0", "url": "https://www.edgepedia.org/edgepedia/license", "summary": "Free with credit, commercial use included. AI training is open to everyone. For other uses, organizations over USD 100M in revenue or 100M monthly users license separately.", "spdx": "LicenseRef-Edgepedia-Community-1.0" }, "credit": "\"Stopped-flow spectroscopy\", Edgepedia (EdgeChat), https://www.edgepedia.org/stopped-flow-spectroscopy. Edgepedia Community License 1.0.", "credit_md": "\"[Stopped-flow spectroscopy](https://www.edgepedia.org/stopped-flow-spectroscopy)\", Edgepedia (EdgeChat), [https://www.edgepedia.org/stopped-flow-spectroscopy](https://www.edgepedia.org/stopped-flow-spectroscopy). [Edgepedia Community License 1.0](https://www.edgepedia.org/edgepedia/license).", "credit_html": "\"Stopped-flow spectroscopy\", Edgepedia (EdgeChat), https://www.edgepedia.org/stopped-flow-spectroscopy. Edgepedia Community License 1.0.", "speakable": "Stopped-flow spectroscopy is a rapid-mixing technique in which reactants are mixed, the flow is abruptly stopped, and the reaction is followed spectroscopically in a fixed cell." }