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 "slug": "x-ray-nanotomography",
 "title": "X-ray nanotomography",
 "updated": "2026-09-30",
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 "excerpt": "X-ray nanotomography, or nano-CT, is a non-destructive imaging method that maps a sample's internal structure in 3D with 400 nm precision or better, bridging micro-CT and electron microscopy.",
 "snippet": "X-ray nanotomography, or nano-CT, is a non-destructive imaging method that maps a sample's internal structure in 3D with 400 nm precision or better, bridging micro-CT and electron microscopy.",
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 "markdown": "# X-ray nanotomography\n\nX-ray nanotomography (nano-CT) is a non-destructive imaging method that maps a sample's X-ray optical density into three-dimensional coordinates with a precision of 400 nm or better, and well below that on the best instruments.<sup>[1](https://journals.iucr.org/s/issues/2026/02/00/gy5084/)</sup> It produces quantitative 3D images of internal structure at length scales between conventional micro-CT, which resolves micrometers, and electron microscopy, which resolves below 3 nm but only in very small, often destructive volumes.<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup><sup> • </sup><sup>[3](https://www.cambridge.org/core/journals/mrs-bulletin/article/nanoscale-xray-and-electron-tomography/ED04F1C6808ADF7EBB37D3133EA2C552)</sup> Like all X-ray computed tomography, it reconstructs 3D images from 2D radiographs acquired at synchrotrons or with laboratory X-ray sources, using absorption or phase contrast.<sup>[4](https://www.nature.com/articles/s43586-021-00015-4)</sup>\n\n| Key fact | Value |\n|---|---|\n| Definition | Mapping of X-ray optical density to 3D coordinates with ≤400 nm precision<sup>[1](https://journals.iucr.org/s/issues/2026/02/00/gy5084/)</sup> |\n| Laboratory resolution | Down to 50 nm (TXM optics); 150–185 nm for lensless projection systems<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup><sup> • </sup><sup>[5](https://www.mdpi.com/2073-4352/11/6/677)</sup> |\n| Synchrotron resolution | ~30 nm TXM tomography in 1 min; 16 nm ptychographic; 4.2 nm half-pitch with burst ptychography<sup>[3](https://www.cambridge.org/core/journals/mrs-bulletin/article/nanoscale-xray-and-electron-tomography/ED04F1C6808ADF7EBB37D3133EA2C552)</sup><sup> • </sup><sup>[6](https://doi.org/10.1038/srep03857)</sup><sup> • </sup><sup>[7](https://doi.org/10.1038/s41586-024-07615-6)</sup> |\n| Acquisition time | Synchrotron 5–30 min; laboratory 1–2 days for comparable image quality<sup>[1](https://journals.iucr.org/s/issues/2026/02/00/gy5084/)</sup> |\n| Contrast modes | Absorption and Zernike phase (\\( \\pi/2 \\) or \\( 3\\pi/2 \\) phase shift); quantitative electron density in ptychographic CT<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup><sup> • </sup><sup>[8](https://www.nature.com/articles/s41467-026-73738-1)</sup> |\n| Sample constraint | Less than 50 µm for 50 nm resolution with 1000–2000-pixel detectors<sup>[9](https://www.sciencedirect.com/science/article/pii/S136970210770305X)</sup> |\n\n## How it works\n\nThree optical principles deliver nanometer resolution. In full-field transmission [X-ray microscopy](https://www.edgepedia.org/x-ray-microscopy) ([TXM](https://www.edgepedia.org/transmission-x-ray-microscopy)), a [Fresnel zone](https://www.edgepedia.org/fresnel-zone) plate (FZP) forms a real image, and resolution follows Abbe's law from the outermost zone width: \\( \\delta_{t} = 0.61\\lambda/\\mathrm{NA} = 1.22 \\Delta R_{n} \\), with depth of focus \\( \\delta_{l} = \\pm 2 \\Delta R_{n}^{2}/\\lambda \\).<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup> In conventional geometric projection-magnification systems, below about 500 nm resolution the projection geometry becomes impractical and lens-based architectures are required; ptychographic and other lensless methods are not subject to this limit and can achieve substantially finer resolution.<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup> In lensless projection magnification, resolution is roughly the X-ray source size, typically limited to about 250 nm because no lens collects the beam within a finite numerical aperture.<sup>[10](https://onlinelibrary.wiley.com/doi/10.1002/0471266965.com133)</sup> In ptychographic X-ray computed tomography (PXCT), a scanning coherent probe and overlapping diffraction patterns reconstruct the complex transmissivity without imaging lenses; resolution is limited not by optics but by the recovered scattering signal and available coherent flux, and can in principle approach the wavelength limit.<sup>[8](https://www.nature.com/articles/s41467-026-73738-1)</sup>\n\nPhase contrast is what makes light elements visible. A Zernike phase ring in the back focal plane of the objective shifts the undiffracted beam by \\( \\pi/2 \\) or \\( 3\\pi/2 \\), so image intensity varies linearly with the sample's phase shift.<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup><sup> • </sup><sup>[9](https://www.sciencedirect.com/science/article/pii/S136970210770305X)</sup> This suits weakly absorbing samples: a magnesium composite with 10 vol% SiC particles absorbs only 5.7% of 11 keV light in a 20 µm sample, yet Zernike contrast resolves its structure.<sup>[11](https://journals.iucr.org/s/issues/2020/05/00/mo5217/mo5217.pdf)</sup>\n\n## How it is done\n\nSample preparation sets the achievable resolution. Sample thickness should be at most about twice the X-ray absorption length at the chosen energy (5.4 or 8 keV in laboratory TXM tools).<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup> TXM ptychographic work often uses FIB-milled pillars, for example 10 µm diameter.<sup>[12](https://doi.org/10.1038/nature21698)</sup> Organic specimens such as cells and tissue are imaged under cryogenic conditions to limit radiation damage.<sup>[13](https://asset-downloads.zeiss.com/catalogs/download/mic/8a7f14f0-d2b9-4102-a929-14b89e44fc00/EN_44_011_023_xradia-synchrotron-family_rel2-0.pdf)</sup>\n\nAcquisition collects hundreds to thousands of projections over 180° or 360°; a Zernike phase nano-[CT scan](https://www.edgepedia.org/ct-scan) of an unstained cell collected 251 projections over 180° within 10 minutes.<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup><sup> • </sup><sup>[14](https://journals.aps.org/prb/abstract/10.1103/PhysRevB.81.140105)</sup> [Reconstruction](https://www.edgepedia.org/reconstruction) uses filtered backprojection or FDK, often via the ASTRA toolbox, with prior geometry calibration and correction of drift and pixel sensitivity irregularities; Gridrec with a Shepp–Logan filter (TomoPy) is common at beamlines, and iterative schemes such as SIRT or SART with self-alignment and total-variation denoising are widely used.<sup>[1](https://journals.iucr.org/s/issues/2026/02/00/gy5084/)</sup><sup> • </sup><sup>[11](https://journals.iucr.org/s/issues/2020/05/00/mo5217/mo5217.pdf)</sup><sup> • </sup><sup>[15](https://iopscience.iop.org/article/10.1088/1748-0221/19/10/P10021/pdf)</sup> Projection data follow Beer–Lambert attenuation, \\( p = -\\ln I/I_{0} \\).<sup>[16](https://pubs.rsc.org/en/content/articlehtml/2024/na/d3na01089a)</sup> [Resolution](https://www.edgepedia.org/resolution) is assessed by Fourier ring correlation or Fourier shell correlation with the half-bit criterion.<sup>[11](https://journals.iucr.org/s/issues/2020/05/00/mo5217/mo5217.pdf)</sup><sup> • </sup><sup>[15](https://iopscience.iop.org/article/10.1088/1748-0221/19/10/P10021/pdf)</sup>\n\n## Origin\n\nThe enabling physics came from phase-contrast imaging of weakly absorbing materials with hard X-rays, reported in 1995 by T. J. Davis and colleagues in Nature, and independently that year by A. Snigirev and colleagues in Review of Scientific Instruments.<sup>[17](https://doi.org/10.1038/373595a0)</sup><sup> • </sup><sup>[18](https://doi.org/10.1063/1.1146073)</sup> P. Cloetens, W. Ludwig, J. Baruchel, and colleagues introduced holotomography, quantitative phase tomography with hard synchrotron radiation, in 1999 in [Applied Physics Letters](https://www.edgepedia.org/applied-physics-letters).<sup>[19](https://doi.org/10.1063/1.125225)</sup> D. Paganin, S. C. Mayo, T. E. Gureyev, and colleagues published the single-distance phase retrieval method in 2002 in the Journal of Microscopy, now the most widely used phase retrieval in propagation-based phase-contrast CT.<sup>[20](https://doi.org/10.1046/j.1365-2818.2002.01010.x)</sup> X-ray nanotomography as a consolidated method was reviewed by Philip J. Withers in a 2007 Materials Today review.<sup>[21](https://doi.org/10.1016/s1369-7021%2807%2970305-x)</sup><sup> • </sup><sup>[30](https://pubmed.ncbi.nlm.nih.gov/10991224/)</sup> In 2010, Marco Stampanoni, Rajmund Mokso, Federica Marone, and colleagues reported Zernike phase-contrast nano-CT at 144 nm isotropic resolution in [Physical Review B](https://www.edgepedia.org/physical-review-b),<sup>[14](https://journals.aps.org/prb/abstract/10.1103/PhysRevB.81.140105)</sup> and Martin Dierolf, Andreas Menzel, Pierre Thibault, and colleagues introduced ptychographic X-ray computed tomography in Nature.<sup>[22](https://doi.org/10.1038/nature09419)</sup>\n\n## Variants\n\n**TXM-based nano-CT** uses a capillary condenser, FZP objective, and optional Zernike phase ring; laboratory versions reach 50 nm and synchrotron versions about 30 nm.<sup>[2](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)</sup><sup> • </sup><sup>[3](https://www.cambridge.org/core/journals/mrs-bulletin/article/nanoscale-xray-and-electron-tomography/ED04F1C6808ADF7EBB37D3133EA2C552)</sup> **Holotomography and X-ray holographic nanotomography (XNH)** use propagation-based phase contrast; the ID16A endstation operates at 17.1 or 33.3 keV in a vacuum chamber evacuated to \\( 1.0 \\times 10^{-7} \\) mbar.<sup>[23](https://pmc.ncbi.nlm.nih.gov/articles/PMC12945482/)</sup> **PXCT** exists in far-field and near-field forms; near-field ptychotomography is quantitative and non-destructive, and the VortRem algorithm removes the phase-vortex artifacts frequent in holographic methods.<sup>[24](https://journals.aps.org/prapplied/abstract/10.1103/PhysRevApplied.14.064078)</sup> **Laminography**, introduced for limited-access geometry by S Gondrom, J Zhou, M Maisl, and colleagues in 1999, rotates the sample about an axis perpendicular to its layers; in ptychographic form this allows arbitrarily extended flat samples such as whole chips, removing the need for cylindrical FIB pillars.<sup>[25](https://doi.org/10.1016/s0029-5493%2898%2900319-7)</sup><sup> • </sup><sup>[12](https://doi.org/10.1038/nature21698)</sup> **Laboratory lensless projection nano-CT** with a 150 nm nano-focus source achieves about 170–185 nm 3D resolution even in high-density materials.<sup>[5](https://www.mdpi.com/2073-4352/11/6/677)</sup> **Scanning nanoprobe tomography** combines nanobeams with scanning schemes to obtain multispectral 3D volumes in a single non-destructive analysis.<sup>[26](https://www.osti.gov/biblio/22053571)</sup> Burst ptychography achieved 4 nm resolution at a 170-times faster acquisition rate, 14,000 resolution elements per second, with tomographic back-propagation allowing samples up to ten times larger than the conventional depth of field.<sup>[7](https://doi.org/10.1038/s41586-024-07615-6)</sup> Dynamic sparse in situ PXCT reconstructs full high-resolution tomograms from 10 projections per time point instead of the roughly 1250 needed by analytic methods for an 80 µm sample at 100 nm resolution, a greater than 100-fold gain in temporal resolution.<sup>[8](https://www.nature.com/articles/s41467-026-73738-1)</sup>\n\n## Applications\n\nIn materials science, nano-CT is routine for battery electrodes: synchrotron TXM imaged nanometer-thick film electrodes on 3D silicon scaffolds in 85 min at sub-10 nm resolution,<sup>[27](https://onlinelibrary.wiley.com/doi/10.1002/adma.202008653)</sup> and representative elementary volumes of 102 × 102 × 102 µm³ at 50 nm voxel size suffice for six anode and cathode chemistries (Gr, Si, LTO, LFP, LMO, NMC).<sup>[28](https://pubs.acs.org/doi/10.1021/acsnano.4c16419)</sup> PXCT imaged integrated circuits non-destructively down to 14.6 nm lateral resolution, resolving individual transistors in a 7-nm-node commercial processor.<sup>[12](https://doi.org/10.1038/nature21698)</sup><sup> • </sup><sup>[7](https://doi.org/10.1038/s41586-024-07615-6)</sup> Other uses include catalysts, fuel-cell electrodes, composites, and soft and hard tissue.<sup>[13](https://asset-downloads.zeiss.com/catalogs/download/mic/8a7f14f0-d2b9-4102-a929-14b89e44fc00/EN_44_011_023_xradia-synchrotron-family_rel2-0.pdf)</sup> In biology, XNH is applied to neuronal tissue, and Zernike nano-CT resolves nucleus and intracellular structures in unstained cells.<sup>[23](https://pmc.ncbi.nlm.nih.gov/articles/PMC12945482/)</sup><sup> • </sup><sup>[14](https://journals.aps.org/prb/abstract/10.1103/PhysRevB.81.140105)</sup>\n\n## Limitations and alternatives\n\nA round-robin comparison found laboratory nano-CT image quality comparable to synchrotron results, but laboratory measurements take 1–2 days while the same measurements at a synchrotron complete within 5–30 minutes.<sup>[1](https://journals.iucr.org/s/issues/2026/02/00/gy5084/)</sup> Exposure time scales inversely with the fourth power of spatial resolution, so laboratory tomography significantly better than 30 nm is likely restricted to synchrotron sources.<sup>[9](https://www.sciencedirect.com/science/article/pii/S136970210770305X)</sup> With 1000–2000-pixel detectors, the sample must be smaller than 50 µm for 50 nm resolution, a serious constraint for engineering samples.<sup>[9](https://www.sciencedirect.com/science/article/pii/S136970210770305X)</sup> [Radiation damage](https://www.edgepedia.org/radiation-damage) is a recognized limitation of high-resolution X-ray CT,<sup>[4](https://www.nature.com/articles/s43586-021-00015-4)</sup> and operando battery work must balance dose to electrolyte and material against reliable electrochemical monitoring.<sup>[28](https://pubs.acs.org/doi/10.1021/acsnano.4c16419)</sup> [Laboratory](https://www.edgepedia.org/laboratory) polychromatic CT suffers beam-hardening cupping artifacts that synchrotron monochromatic radiation avoids.<sup>[29](https://www.ndt.net/article/ecndt2010/reports/1_04_24.pdf)</sup> Compared with electron tomography, which resolves below 3 nm but is constrained by the Crowther limit \\( n = \\pi D/d \\) and almost always by radiation damage, nano-CT images bulk materials in 3D thanks to X-ray penetrating power.<sup>[3](https://www.cambridge.org/core/journals/mrs-bulletin/article/nanoscale-xray-and-electron-tomography/ED04F1C6808ADF7EBB37D3133EA2C552)</sup> FIB-SEM serial sectioning and electron-microscope-based nano-CT are destructive or require vacuum-resistant, conductive samples, which transmission-target X-ray sources avoid.<sup>[5](https://www.mdpi.com/2073-4352/11/6/677)</sup> Conventional micro-CT operates at micrometer resolution with the same beam-hardening limits.<sup>[29](https://www.ndt.net/article/ecndt2010/reports/1_04_24.pdf)</sup>\n\n## References\n\n1. [Comparing image quality of synchrotron and laboratory nano-CT scans: a round robin study (IUCrJ, 2026)](https://journals.iucr.org/s/issues/2026/02/00/gy5084/)\n2. [X-ray Nanotomography in the Laboratory with ZEISS Xradia Ultra 3D X-ray Microscopes](https://asset-downloads.zeiss.com/catalogs/download/mic/e9d1aa39-51ea-4150-bf16-4120b22580c2/EN_wp_x-ray-nanotomography_Xradia-Ultra.pdf)\n3. [Nanoscale x-ray and electron tomography (MRS Bulletin)](https://www.cambridge.org/core/journals/mrs-bulletin/article/nanoscale-xray-and-electron-tomography/ED04F1C6808ADF7EBB37D3133EA2C552)\n4. [X-ray computed tomography (Nature Reviews Methods Primers, 2021)](https://www.nature.com/articles/s43586-021-00015-4)\n5. [Laboratory-Based Nano-Computed Tomography and Examples of Its Application in the Field of Materials Research (Crystals, 2021; published version of the ntCT arXiv paper)](https://www.mdpi.com/2073-4352/11/6/677)\n6. [M. Holler and colleagues (2014). X-ray ptychographic computed tomography at 16 nm isotropic 3D resolution. Scientific Reports.](https://doi.org/10.1038/srep03857)\n7. [Tomas Aidukas and colleagues (2024). High-performance 4-nm-resolution X-ray tomography using burst ptychography. Nature.](https://doi.org/10.1038/s41586-024-07615-6)\n8. [In situ ptychographic x-ray nanotomography of temperature-controlled crystallization processes (Nature Communications, 2026)](https://www.nature.com/articles/s41467-026-73738-1)\n9. [X-ray nanotomography (Withers, Materials Today 10, 23–34, 2007)](https://www.sciencedirect.com/science/article/pii/S136970210770305X)\n10. [Characterization of Materials (Wiley reference work)](https://onlinelibrary.wiley.com/doi/10.1002/0471266965.com133)\n11. [Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments (Journal of Synchrotron Radiation, 2020)](https://journals.iucr.org/s/issues/2020/05/00/mo5217/mo5217.pdf)\n12. [Mirko Holler and colleagues (2017). High-resolution non-destructive three-dimensional imaging of integrated circuits. Nature.](https://doi.org/10.1038/nature21698)\n13. [ZEISS Xradia Synchrotron Family Nanoscale X-ray Microscopy](https://asset-downloads.zeiss.com/catalogs/download/mic/8a7f14f0-d2b9-4102-a929-14b89e44fc00/EN_44_011_023_xradia-synchrotron-family_rel2-0.pdf)\n14. [Phase-contrast tomography at the nanoscale using hard x rays (Stampanoni et al., Phys. Rev. B 81, 140105, 2010)](https://journals.aps.org/prb/abstract/10.1103/PhysRevB.81.140105)\n15. [Laboratory x-ray nano-computed tomography for biomedical samples (JINST 19, P10021, 2024)](https://iopscience.iop.org/article/10.1088/1748-0221/19/10/P10021/pdf)\n16. [Improving reconstructions in nanotomography for homogeneous materials via mathematical optimization (Nanoscale Advances, 2024)](https://pubs.rsc.org/en/content/articlehtml/2024/na/d3na01089a)\n17. [T. J. Davis and colleagues (1995). Phase-contrast imaging of weakly absorbing materials using hard X-rays. Nature.](https://doi.org/10.1038/373595a0)\n18. [A. Snigirev and colleagues (1995). On the possibilities of x-ray phase contrast microimaging by coherent high-energy synchrotron radiation. Review of Scientific Instruments.](https://doi.org/10.1063/1.1146073)\n19. [P. Cloetens and colleagues (1999). Holotomography: Quantitative phase tomography with micrometer resolution using hard synchrotron radiation x rays. Applied Physics Letters.](https://doi.org/10.1063/1.125225)\n20. [D. Paganin and colleagues (2002). Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object. Journal of Microscopy.](https://doi.org/10.1046/j.1365-2818.2002.01010.x)\n21. [X-ray nanotomography (Materials Today, 2007)](https://doi.org/10.1016/s1369-7021%2807%2970305-x)\n22. [Martin Dierolf and colleagues (2010). Ptychographic X-ray computed tomography at the nanoscale. Nature.](https://doi.org/10.1038/nature09419)\n23. [Scaling up X-ray holographic nanotomography for neuronal tissue imaging (PMC)](https://pmc.ncbi.nlm.nih.gov/articles/PMC12945482/)\n24. [Phase-Vortex Removal for Quantitative X-Ray Nanotomography with Near-Field Ptychography (Zanette et al., Phys. Rev. Applied, 2020)](https://journals.aps.org/prapplied/abstract/10.1103/PhysRevApplied.14.064078)\n25. [X-ray computed laminography: an approach of computed tomography for applications with limited access (Nuclear Engineering and Design, 1999)](https://doi.org/10.1016/s0029-5493%2898%2900319-7)\n26. [A hard x-ray nanoprobe for scanning and projection nanotomography](https://www.osti.gov/biblio/22053571)\n27. [Fast X-ray Nanotomography with Sub-10 nm Resolution as a Powerful Imaging Tool for Nanotechnology and Energy Storage Applications (De Andrade et al., Advanced Materials, 2021)](https://onlinelibrary.wiley.com/doi/10.1002/adma.202008653)\n28. [Comparative Study of the Quantitative Analysis of Battery Materials with X-ray Nano-tomography: From Ex Situ toward Operando Measurements (Vanpeene et al., ACS Nano 19(10): 9994–10012, 2025)](https://pubs.acs.org/doi/10.1021/acsnano.4c16419)\n29. [Comparison Between X-Ray-Tube Based and Synchrotron Based µCT (ECNDT 2010)](https://www.ndt.net/article/ecndt2010/reports/1_04_24.pdf)\n30. [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/10991224/)\n\n---\n*Topic: Encyclopedia › Physical world and mathematics › Physics › Physics methods, practice, and community › X-ray imaging and tomography*\n\n*Initially written Sep 29, 2026 · Reviewed: Sep 30, 2026 · Edited: Sep 30, 2026 · Last review: Sep 30, 2026*\n\n*Copyright 2026 EdgeChat AI, a subsidiary of Biostate AI.*\n\nLicense: Edgepedia Community License 1.0, https://www.edgepedia.org/edgepedia/license\n",
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 "credit_md": "\"[X-ray nanotomography](https://www.edgepedia.org/x-ray-nanotomography)\", Edgepedia (EdgeChat), [https://www.edgepedia.org/x-ray-nanotomography](https://www.edgepedia.org/x-ray-nanotomography). [Edgepedia Community License 1.0](https://www.edgepedia.org/edgepedia/license).",
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 "speakable": "X-ray nanotomography, or nano-CT, is a non-destructive imaging method that maps a sample's internal structure in 3D with 400 nm precision or better, bridging micro-CT and electron microscopy."
}
