9 articles
Barnase
Barnase, a 110-residue guanyl-specific ribonuclease secreted by Bacillus amyloliquefaciens, is neutralized by its 89-residue inhibitor barstar; the pair is used in plant male sterility, biosafety kill switches, and cancer research.
CRISPR-associated protein Cas6/Cmx6
Cas6/Cmx6, a CRISPR-associated RAMP endoribonuclease with two ferredoxin-like RRM domains, metal-independently cleaves pre-crRNA repeats in type I and III systems, making mature guide RNAs; binds 50 pM-10 nM, turns over 0.5-3 min-1.
Pancreatic ribonuclease
Pancreatic ribonuclease, also called ribonuclease A (RNase A) or RNase 1, is a digestive enzyme that breaks down RNA, encoded in humans by the RNASE1 gene.
Ribonuclease
Ribonuclease (RNase), enzymes that cleave RNA phosphodiester bonds; RNase A gave the first enzyme mechanism (Mathias and Rabin, 1961) using His12/His119 general acid-base catalysis, while RNase H and RNase III are metal-dependent families.
Ribonuclease ageritin
Ribonuclease ageritin (Agt1) is a metal-dependent ribotoxin-like protein from the edible poplar mushroom Cyclocybe aegerita, the first basidiomycete ribotoxin; it cleaves the sarcin-ricin loop of 28S rRNA and shows antifungal, entomotoxic, and antitumor activities.
Ribonuclease III
Ribonuclease III (RNase III, EC 3.1.26.3, ribonuclease 3) is a Mg2+-dependent endonuclease that cleaves double-stranded RNA, leaving 2-nt 3′ overhangs; the E. coli rnc enzyme is the prototype of a family including Dicer and Drosha.
Ribonuclease P, Rpm2, mitochondrial
Rpm2 (RPM2, Rpm2p) is the 105-kDa nuclear-encoded protein subunit of Saccharomyces cerevisiae mitochondrial RNase P, paired with the mitochondrial Rpm1 RNA, with extra roles in translation, transcription, and P bodies.
Ribonuclease ribotoxin, alpha-sarcin type
Ribonuclease ribotoxin, alpha-sarcin type: fungal cytotoxic RNases (~150 aa, 17 kDa alpha-sarcin from Aspergillus giganteus) that cleave one bond in the sarcin-ricin loop of 28S rRNA, halting translation; relatives restrictocin, mitogillin, Aspf1, hirsutellin A.
Ribonuclease T
Ribonuclease T (RNase T, rnt gene product) is a 23 kDa DEDD-family 3'→5' exoribonuclease of E. coli that matures 5S/23S rRNA and tRNA ends, stops at CC residues, and also acts as a single-strand DNase.