41 articles
Reconstitution assay
A reconstitution assay, or reconstitution experiment, is a biochemical method that rebuilds a biological process from purified components to identify the minimal set of factors it requires.
Resonance energy transfer
Förster resonance energy transfer (FRET) is a spectroscopic technique measuring nanometer-scale distances between two fluorophores by nonradiative transfer of excitation energy from donor to acceptor.
Respirometry
Respirometry is a measurement technique that quantifies how fast an organism, tissue, or cell consumes oxygen to estimate metabolic rate, making it a form of indirect calorimetry.
Reverse phase protein array
Reverse phase protein array (RPPA) is a targeted proteomics method that spots many samples as microdots on slides and probes them one antibody at a time.
RNA Bind-n-Seq
RNA Bind-n-Seq (RBNS) is an in vitro assay, introduced in 2014, that measures the binding specificity of RNA-binding proteins across random RNA sequences using deep sequencing.
Scintillation proximity assay
Scintillation proximity assay (SPA) is a bead-based radioisotope assay in which a radiolabeled ligand emits light only when bound near a scintillant bead, requiring no separation step.
SOMAscan assay
The SOMAscan assay, also written SomaScan, is an aptamer-based proteomics platform that measures about 11,000 human proteins from small serum or plasma samples, using modified DNA aptamers called SOMAmers.
Supramolecular tandem assay
A supramolecular tandem assay is a label-free method for monitoring enzyme activity in real time, using a macrocyclic host and fluorescent dye whose displacement reports the enzymatic conversion.
Telomeric repeat amplification protocol
The telomeric repeat amplification protocol (TRAP) is a PCR-based assay that detects telomerase activity in cell extracts, widely used in cancer and aging research since 1994.
Thermal shift assay
A thermal shift assay (TSA), also called differential scanning fluorimetry or ThermoFluor, measures protein thermal stability by heating it and detecting unfolding with a fluorescent dye.
Western blot
Western blot (immunoblot): antibody-based technique detecting specific proteins after SDS-PAGE separation and membrane transfer, introduced 1979 by Towbin, named by Burnette 1981; semi-quantitative, size-resolving, distinct from ELISA.