11 articles
Anchorage-independent growth assay
The anchorage-independent growth assay tests whether cells can proliferate without attachment to a surface, most commonly in soft agar, and serves as a stringent in vitro test of malignant transformation.
Cell migration assay
A cell migration assay is a laboratory method that measures how cells move, in formats such as wound healing (scratch) and transwell chambers, used to test chemoattractants, drugs, and gene perturbations.
Cell Migration Assays, Macrophage
Macrophage cell migration assays: capillary migration inhibition tests measuring delayed hypersensitivity via MIF, and chemotaxis formats (Boyden/transwell, Dunn chamber, under-agarose, microfluidics) measuring directed macrophage movement at about 10 µm/min.
Cell Painting
Cell Painting is a fixed-cell, high-content imaging assay introduced in 2013 that stains cells with six fluorescent dyes to produce morphological profiles used in drug discovery.
Cell viability assay
A cell viability assay is a bench biology method that estimates the proportion of living cells in a culture, usually through a metabolic, dye-based, or ATP readout.
Clonogenic assay
The clonogenic assay measures whether individual cells can proliferate into colonies of 50 or more cells, the in vitro gold standard for testing radiotherapy and chemotherapy sensitivity.
Hemolysis assay
A hemolysis assay is a bench biology method that measures rupture of red blood cells by spectrophotometrically detecting the hemoglobin they release, reporting membrane damage as a hemolysis percentage.
High-content screening
High-content screening is a cell biology method combining automated microscopy and image analysis to measure many features of individual cells, introduced in 1997 for drug discovery.
Optical pooled screening
Optical pooled screening is a cell-biology method that applies pooled perturbations to a mixed cell population, images each cell's phenotype, and identifies each cell's perturbation in place.
Scratch assay
The scratch assay, also called the wound-healing assay, measures cell migration by creating a gap in a confluent cell monolayer and imaging cells migrating in to close it.
Soft agar assay
The soft agar assay is a cell biology method that grows cells suspended in semi-solid agar to test anchorage-independent growth, serving as an in vitro proxy for tumorigenic potential.