6 articles
Alkaline lysis
Alkaline lysis is a molecular biology method, introduced by Birnboim and Doly in 1979, that breaks open bacterial cells to release plasmid DNA separate from chromosomal DNA.
Cell disruption
Cell disruption is the laboratory step of permeating or breaking open cells to release proteins, nucleic acids, or metabolites, by methods classified as physical, chemical, or mechanical.
Cell fractionation
Cell fractionation is a cell biology method that breaks cells open and separates organelles, membranes, and proteins into enriched fractions by centrifugation, enabling analysis of cellular compartments.
Differential centrifugation
Differential centrifugation is a cell fractionation method that separates cell components by spinning a homogenate at stepwise increasing speeds, pelleting nuclei, mitochondria, and microsomes in sequence.
Spheroplast
Spheroplasts: bacteria or yeast that have partially lost their cell wall and become round; wall-less Gram-negative forms retaining an outer membrane, used in patch clamp, imaging, transformation, and DNA delivery.
Tissue dissociation
Tissue dissociation is a laboratory method that breaks solid tissue into a suspension of single living cells, combining enzymatic digestion with mechanical shearing for analysis or culture.