13 articles
Cell cloning
Cell cloning is a bench biology method that expands a single isolated cell into a genetically uniform cell line, used in hybridoma production, recombinant protein development, and gene editing.
Cell fusion
Cell fusion is the merging of two or more cells into a single cell, occurring naturally during development and induced in the laboratory to make hybridomas.
Cell Separation
Cell separation (cell isolation, cell sorting) comprises techniques for separating distinct cell populations, including density fractionation, FACS, MACS, and microfluidic methods, used in immunology, diagnostics, and cell therapy manufacturing.
Cell sorting
Cell sorting is a bench biology technique that separates cells from a mixed suspension by physical or fluorescent properties, most commonly by flow cytometry, to produce purified cell populations.
Chromosome transfer
Chromosome transfer is a somatic cell genetics technique that moves intact chromosomes from donor cells into recipient cells, most often by microcell-mediated chromosome transfer (MMCT).
Dilution cloning
Dilution cloning, or limiting dilution cloning, is a cell biology technique that isolates single cells by serial dilution into microtiter plate wells and grows clonal cell lines from them.
Immunomagnetic separation
Immunomagnetic separation (IMS) is a bench technique that uses antibody-coated magnetic beads to capture target cells, bacteria, or vesicles, underlying commercial platforms such as Dynabeads and MACS.
Laser capture microdissection
Laser capture microdissection, also called laser microdissection, is a technique that uses a laser to isolate specific cells from stained tissue sections for DNA, RNA, and protein analysis.
Microdissection
Microdissection is a laboratory technique that uses a microscope to isolate specific cells or tissue regions from a section for DNA, RNA, or protein analysis.
Microinjection
Microinjection is a laboratory technique that uses a fine glass needle, guided by a micromanipulator under a microscope, to inject DNA, RNA, or proteins into a living cell.
Micromanipulation
Micromanipulation is a laboratory technique using fine glass microtools on remotely controlled arms under a microscope to handle individual cells and embryos, central to assisted reproduction, cloning, and single-cell biology.
Negative selection (cell separation)
Negative selection, also called negative cell selection, is a cell separation method in which unwanted cells are antibody-tagged and removed, leaving desired cells unbound and unmanipulated.
Stem cell isolation
Stem cell isolation is the set of laboratory methods for separating rare stem cells from mixed populations in tissues, blood, or cultures, producing enriched cells for transplantation and research.