11 articles
Automated cell counting
Automated cell counting is a bench biology technique that uses image analysis or impedance instruments to measure cell concentration and viability, replacing manual microscopy with a hemocytometer.
Cytophotometry
Cytophotometry: microscope-based quantification of stained or fluorescent cell components, chiefly Feulgen-stained DNA, founded on Caspersson's 1930s microspectrophotometry; static single-cell ancestor of flow and image cytometry.
Hemocytometry
Hemocytometry is a bench microscopy technique that counts cells in a known volume in a calibrated chamber, giving cell concentration and, with a vital dye, viability.
Image cytometry
Image cytometry is a cell-analysis method in microscopy that records images of individual cells and derives per-cell fluorescence, localization, size, shape, and texture measurements, unlike conventional flow cytometry.
Imaging flow cytometry
Imaging flow cytometry (IFC) combines the high event rates of flow cytometry with single-cell imaging, capturing up to 12 images per cell and calculating hundreds of features.
Imaging mass cytometry
Imaging mass cytometry (IMC) is a tissue imaging method that uses metal-isotope-labeled antibodies and mass spectrometry to measure over 40 proteins at 1 µm resolution, introduced in 2014.
Impedance cytometry
Impedance cytometry is a label-free flow cytometry technique that measures the electrical impedance of individual cells passing through a microfluidic aperture, quantifying cell size and dielectric properties without stains or optics.
Laser scanning cytometry
Laser scanning cytometry (LSC) is a slide-based technique in which lasers scan cells on a microscope slide, producing per-cell fluorescence data comparable to flow cytometry.
Mass cytometry
Mass cytometry, known commercially as CyTOF, is a single-cell technique introduced in 2009 that measures over 40 cellular parameters with heavy-metal-tagged antibodies, used mainly for immune profiling.
Next-generation flow cytometry
Next-generation flow cytometry, also called full-spectrum or spectral flow cytometry, measures each cell's full fluorescence emission spectrum, allowing panels of 35 or more antigens in one tube.
Spectral flow cytometry
Spectral flow cytometry is a single-cell analysis technique that measures the full fluorescence spectrum of each cell, separating dyes by spectral unmixing in panels of up to 50 colors.