6 articles
MERFISH
MERFISH (multiplexed error-robust fluorescence in situ hybridization) is an imaging method measuring the copy numbers and spatial locations of hundreds to thousands of RNA species in single cells.
Multiplex fluorescence in situ hybridization
Multiplex fluorescence in situ hybridization (multiplex FISH) is a microscopy method that uses fluorescent probes to detect hundreds of RNA or DNA targets in fixed cells and tissue.
RNA fluorescence in situ hybridization
RNA fluorescence in situ hybridization (RNA FISH) detects and locates specific RNA molecules in fixed cells with fluorescent probes, counting individual transcripts and revealing their subcellular positions.
seqFISH
seqFISH (sequential fluorescence in situ hybridization) is an imaging-based spatial transcriptomics method that localizes RNA in fixed cells; its seqFISH+ variant images 10,000 genes per cell.
Sequential fluorescence in situ hybridization
Sequential fluorescence in situ hybridization (seqFISH) is a multiplexed RNA imaging method that repeatedly hybridizes, images, and strips probes to barcode transcripts in fixed cells.
Single-molecule fluorescence in situ hybridization
Single-molecule fluorescence in situ hybridization (smFISH) is a molecular biology method that counts individual RNA molecules in fixed cells using many short fluorescent probes, making each transcript a visible spot.