11 articles
Brainbow
Brainbow is a genetic labeling technique, reported by Jean Livet and colleagues at Harvard in 2007, that uses Cre-lox recombination to give neighboring neurons different fluorescent colors, allowing their axons to be traced individually.
DNA transfection
DNA transfection is a laboratory method that introduces foreign DNA into cultured cells, typically with chemical reagents or electric pulses, to express proteins or study gene function.
dTAG
dTAG is a targeted protein degradation method in which a small-molecule ligand recruits an E3 ubiquitin ligase to degrade a protein fused to an FKBP12F36V tag, typically within one to two hours.
Fluorescent tagging
Fluorescent tagging is a bench biology method that attaches fluorescent proteins or dyes to biomolecules, letting researchers track their location and dynamics in living cells.
HaloTag
HaloTag is a protein tagging technology in which an engineered 33 kDa bacterial dehalogenase covalently binds chloroalkane ligands, allowing one fusion construct for imaging, purification, and degradation.
mRNA transfection
mRNA transfection is the delivery of synthetic messenger RNA into cultured cells so their ribosomes translate it into protein, giving fast, tunable, transient expression without genomic integration.
Nanoflares
A nanoflare is a DNA-coated gold nanoparticle that enters living cells without transfection agents and reports a specific RNA target by fluorescence, introduced at Northwestern University in 2007.
Protein tagging
Protein tagging is a bench biology method that attaches a detectable label to a protein of interest, either genetically or chemically, to report its location and interactions.
Proximity labeling
Proximity labeling is a molecular biology technique in which an engineered enzyme fused to a protein of interest tags nearby proteins in living cells for mass spectrometry.
Sonoporation
Sonoporation is a delivery technique that uses ultrasound, usually with gas-filled microbubbles, to create transient pores in cell membranes so drugs, genes, proteins, and nanoparticles can enter living cells.
Transient transfection
Transient transfection is a cell biology method that introduces DNA or RNA into cultured cells without genomic integration, producing temporary expression that fades over several days.