6 articles
De novo sequence assembly
De novo sequence assembly is a bioinformatics method that reconstructs complete genome or transcript sequences directly from sequencing reads without a reference genome, producing contigs, scaffolds, or transcripts.
Demultiplexing (sequencing)
Demultiplexing in sequencing is the computational step that separates pooled high-throughput sequencing reads into per-sample FASTQ files by matching each read's index or barcode sequence.
Pairwise sequence alignment
Pairwise sequence alignment arranges two biological sequences, whether DNA, RNA, or protein, into a one-to-one correspondence with gaps, so that similar positions line up; it was first formulated by Needleman and Wunsch in 1970.
Read mapping
Read mapping is the bioinformatics step that aligns short DNA or RNA sequencing reads to a reference genome or transcriptome, with alignments stored in SAM or BAM format.
Smith–Waterman algorithm
The Smith–Waterman algorithm is a dynamic-programming method for finding the optimal local alignment of two biological sequences, published by Temple Smith and Michael Waterman in 1981.
Whole genome alignment
Whole genome alignment is a bioinformatics method that aligns the DNA sequences of complete genomes to identify conserved, rearranged, and diverged regions between species or strains.