6 articles
Amplicon sequencing
Amplicon sequencing is a targeted DNA sequencing method in which PCR-amplified genomic loci are sequenced in depth, used for profiling microbial communities and detecting known or rare genetic variants.
Anchored multiplex polymerase chain reaction
Anchored multiplex polymerase chain reaction (AMP) is a targeted sequencing method, described in 2014 at Massachusetts General Hospital, that detects gene fusions with unknown partners from FFPE specimens.
Capture sequencing
Capture sequencing is a targeted DNA sequencing method that uses biotinylated hybridization probes to enrich selected genomic regions, such as the exome or cancer gene panels, before sequencing.
Exome
The exome is the sequence of all exons in a genome, the roughly 1-2 percent of human DNA that codes for proteins across about 20,000 genes; whole exome sequencing targets it for lower-cost disease-variant discovery than whole genome sequencing.
Hybrid capture
Hybrid capture is a targeted sequencing method that enriches specific genomic regions before sequencing, using biotinylated probes and streptavidin beads; it underlies exome sequencing and custom clinical panels.
Targeted sequencing
Targeted sequencing is a DNA sequencing approach that enriches defined genomic regions, such as an exome or disease-gene panel, before sequencing, producing fewer data at lower cost than whole-genome sequencing.