9 articles
Cryoultramicrotomy
Cryoultramicrotomy is the technique of cutting ultrathin frozen sections with a diamond knife at low temperature, underpinning Tokuyasu immunolabeling and CEMOVIS cryo-electron microscopy of vitreous sections.
Decalcification (histology)
Decalcification is a laboratory procedure that removes calcium salts from bone and calcified tissue, using acids or EDTA, so specimens can be cut into thin sections for microscopic diagnosis.
Diff-Quik stain
Diff-Quik stain is a rapid Romanowsky stain for blood smears and cytology, completing in seconds to minutes what Wright-Giemsa staining takes 20 to 30 minutes.
Frozen section diagnosis
Frozen section diagnosis is a pathology technique in which fresh, unfixed tissue is rapidly frozen, cut, stained, and examined microscopically during surgery, giving the surgeon a preliminary diagnosis within minutes.
Macrodissection
Macrodissection is a pathology laboratory technique in which a pathologist manually removes a marked tumor region from unstained tissue sections, enriching extracted DNA or RNA for molecular testing.
Multiplexed immunohistochemistry
Multiplexed immunohistochemistry (mIHC) stains a single tissue section with several antibodies so two or more biomarkers can be detected in place, typically 2 to 50 markers, underpinning tumor immune profiling and biomarker work in immuno-oncology.
Tissue microarray
A tissue microarray (TMA) is a paraffin block holding hundreds of small tissue cores from donor specimens, allowing parallel analysis of many molecular markers across hundreds of tumors.
Tissue processing
Tissue processing, also called tissue preparation, is the laboratory sequence of dehydration, clearing, and paraffin infiltration that converts fixed tissue into a block for sectioning.
Trichrome stain
The trichrome stain is a histological method using contrasting acid dyes to color collagen blue or green while muscle and cytoplasm stain red, widely used for liver fibrosis.