5 articles
CDNA library
cDNA library: a clone collection of complementary DNA reverse-transcribed from mRNA of a tissue or cell type; contains only expressed, intron-free genes, unlike genomic libraries, for cloning, expression, and sequencing studies.
De-extinction
De-extinction, or proxy species creation, attempts to create organisms resembling extinct species via genome editing, cloning, or back-breeding; IUCN 2016 principles govern it, and Colossal's edited dire wolves (2024-2025) are the first announced case.
Laboratory mouse
The laboratory mouse is a house mouse of defined genetic background bred for biomedical research; its inbred strains, first created by Clarence Little in 1909, allow researchers worldwide to run comparable experiments.
Molecular cloning
Molecular cloning: laboratory methods for isolating and copying a specific DNA segment by inserting it into a self-replicating vector. Also called recombinant DNA cloning; spans restriction-ligation, Golden Gate, Gibson, and Gateway methods, originating with Cohen and Boyer in 1973.
Revival of the woolly mammoth
Revival of the woolly mammoth: Colossal Biosciences' de-extinction project, founded 2021 by Ben Lamm and George Church, editing Asian elephant DNA with CRISPR to create a cold-adapted mammoth-like elephant, proven by the 2025 woolly mouse, birth targeted 2028.