6 articles
Adeno-associated virus
Adeno-associated virus (AAV), a Dependovirus, is a 20–25 nm non-enveloped single-stranded DNA virus with a ~4.7 kb genome; recombinant AAV is the leading in vivo gene therapy vector, with approved products including Luxturna, Zolgensma, and Hemgenix.
Cloning vector
Cloning vector: a DNA molecule, plasmid, phage, cosmid, BAC, or fosmid, that carries foreign DNA into a host cell for replication. Defined by origin of replication, selectable marker, and cloning site; plasmids take up to 12 kb, BACs and fosmids take tens to hundreds of kbp.
Expression vector
Expression vector: DNA vehicle, usually a plasmid or virus, carrying a foreign gene plus promoter, replication origin, and selection marker to produce protein in bacterial, yeast, insect, mammalian, or plant host cells.
PBR322
pBR322 is a 4363-bp E. coli plasmid cloning vector built in 1977 by Bolivar and Rodriguez from pBR313, carrying ampicillin and tetracycline resistance, unique restriction sites, and a pMB1/ColE1-type origin; parent of pUC and pET vectors.
Transfection
Transfection is the deliberate introduction of nucleic acids, such as DNA or mRNA, into cells by non-viral means, used in gene therapy and CAR-T cell therapy.
Vector (molecular biology)
A vector, or vector DNA, is a DNA molecule such as a plasmid or virus used to carry DNA into a host cell for cloning or gene therapy.